LOCUS       sequence-166830-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DONOR vector for Gateway cloning of EWSR1 c1655t No Stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pDONR221-EWSR1 c1655t No Stop
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctggg tcgtagggcc
      121 gatctctgcg ctcctgacgg tgctcgcctt tatccatttt tccaggtcct ccacgtcctc
      181 ctcttcttcc tcccatctgt tccatcaaag gtccaggggg ccccccaggg ccacctcgtc
      241 ttcctccacc aaagccacct cggtccatgc cccggccacc acggaagcca cctctgtctc
      301 caccacggcc acctctgaac attccaccgg gaccaccacg atccatgagg ccacctcttc
      361 ctccccgcat gccaccaggg ccacctctgc cacgatcacc acccgggggc ggaaagggtg
      421 gcaggaggaa gccttcaggc tttggggcct tacactggtt gcactctgtt ctccaggcga
      481 agttctggtt tccacaaccc ggattgggac actgccagtc tccagctcgg tgctggacgt
      541 ttcctcctcc agaggggttc cctcgggaac cccggggtcc tcttggaggg aagcctcctc
      601 tatctcctcc acggcctccc atgcgaccca tgggtccccc aggacctcct gggcctcctg
      661 gacctccacg gagtggtggt ggcatgcctc tgccctcacg gggtggcaga ccaccccgca
      721 tactgttcat tggaggcttc ttccgagcaa gggagacttt aagtttgctc ccttgaaaat
      781 ctttcccatc aaaccattcc acggcagcct tggcagtggg tgggtcttca ta
//
