LOCUS       sequence-166831-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, 
            Kodama A, Ishihara T, Mizushima T, Mizushima N
  TITLE     An Autophagic Flux Probe that Releases an Internal Control.
  JOURNAL   Mol Cell. 2016 Nov 17;64(4):835-849. doi: 
            10.1016/j.molcel.2016.09.037. Epub 2016 Nov 3.
  PUBMED    27818143
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.09.037"; journalName: "Mol Cell"; date: 
            "2016-11-17- 17"; volume: "64"; issue: "4"; pages: "835-849"
COMMENT     Sequence Label: pMRX-IP-GFP-LC3-RFP
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(564..585)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgcg caccttgaag
       61 cgcatgaact ccttgatgac gtcctcggag gaggccatcc cgaacgtctc ctgggaggca
      121 tagaccatgt acaggaagcc gtcttcatct ctctcgctct cgtacacttc agagatgggt
      181 gtggacacac tcaccatgct gtgcccattc accaggagga agaaggcttg gttagcattg
      241 agctgcaggc gccttctaat tatcttgatg agttcgctca tattcacgtg atcaggtaca
      301 aggaacttgg tcttgtccag gacgggcagc tgcttctcac ccttgtatcg ctctataatc
      361 actgggatct tggtggggtg ctgctcccgg atgagccgga catcttccac tctttgttcg
      421 aagctccggc gctgtttgaa ggtcttctcg gacggcatgg aattcaattc atcgatagat
      481 ccgccgccac ccgacccacc accgcccgag ccaccgccac ccttgtacag ctcgtccatg
      541 ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
      601 ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg
      661 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc
      721 tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg
      781 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
      841 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ct
//
