LOCUS       sequence-166832-         313 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, 
            Kodama A, Ishihara T, Mizushima T, Mizushima N
  TITLE     An Autophagic Flux Probe that Releases an Internal Control.
  JOURNAL   Mol Cell. 2016 Nov 17;64(4):835-849. doi: 
            10.1016/j.molcel.2016.09.037. Epub 2016 Nov 3.
  PUBMED    27818143
REFERENCE   2  (bases 1 to 313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.09.037"; journalName: "Mol Cell"; date: 
            "2016-11-17- 17"; volume: "64"; issue: "4"; pages: "835-849"
COMMENT     Sequence Label: pMRX-IP-GFP-LC3-RFP
FEATURES             Location/Qualifiers
     source          1..313
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     141..164
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ccccgaggac ggcgccctga agggcgagat caagatgagg ctgaagctga aggacggcgg
       61 ccactacgac gccgaggtca agaccaccta catggccaag aagcccgtgc agctgcccgg
      121 cgcctacaag accgacatca agctggacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gagcgcgccg agggccgcca ctccaccggc gcctaaagat cccagtgtgg
      241 tggtacggga attcctgcag gcctcgaggg ccggcgcgcc gcggccgcta cgtaaattcc
      301 gccccccccc ccc
//
