LOCUS       sequence-166833-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DONOR vector for Gateway cloning of EWSR1 g1750a Stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pDONR221-EWSR1 g1750a Stop
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
       61 cttttttata atgccaactt tgtacaaaaa agcaggcttc gaaggagata acaaaatggc
      121 gtccacggat tacagtacct atagccaagc tgcagcgcag cagggctaca gtgcttacac
      181 cgcccagccc actcaaggat atgcacagac cacccaggca tatgggcaac aaagctatgg
      241 aacctatgga cagcccactg atgtcagcta tacccaggct cagaccactg caacctatgg
      301 gcagaccgcc tatgcaactt cttatggaca gcctcccact ggttatacta ctccaactgc
      361 cccccaggca tacagccagc ctgtccaggg gtatggcact ggtgcttatg ataccaccac
      421 tgctacagtc accaccaccc aggcctccta tgcagctcag tctgcatatg gcactcagcc
      481 tgcttatcca gcctatgggc agcagccagc agccactgca cctacaagac cgcaggatgg
      541 aaacaagccc actgagacta gtcaacctca atctagcaca gggggttaca accagcccag
      601 cctaggatat ggacagagta actacagtta tccccaggta cctgggagct accccatgca
      661 gccagtcact gcacctccat cctaccctcc taccagctat tcctctacac agccgactag
      721 ttatgatcag agcagttact ctcagcagaa cacctatggg caaccgagca gctatggaca
      781 gcagagtagc tatggtcaac aaagcagcta tgggcagcag cctcccacta gttacccacc
      841 ccaaactgga tcctacagcc aagctccaag tcaatatagc caacagagca gcagc
//
