LOCUS       sequence-166835-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DONOR vector for Gateway cloning of EWSR1 g1750a Stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pDONR221-EWSR1 g1750a Stop
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 agctggatgg caataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gataagcaat gctttcttat aatgccaact ttgtacaaga aagctgggtc ctagtagggc
      121 cgatctctgc gctcctgacg gtgctcgcct ttatccattt ttccaggtcc tccacgtcct
      181 cctcttcttc ctcccatctg ttccatcaaa ggtccagggg gccccccagg gccacctcgt
      241 cttcctccac caaagccacc tcggtccatg ccccggccac cacggaagcc acctctgtct
      301 ccaccacggc cacctctgaa cattccactg ggaccaccac gatccatgag gccacctctt
      361 cctccccgca tgccaccagg gccacctctg ccacgatcac cacccggggg cggaaagggt
      421 ggcgggagga agccttcagg ctttggggcc ttacactggt tgcactctgt tctccaggcg
      481 aagttctggt ttccacaacc cggattggga cactgccagt ctccagctcg gtgctggacg
      541 tttcctcctc cagaggggtt ccctcgggaa ccccggggtc ctcttggagg gaagcctcct
      601 ctatctcctc cacggcctcc catgcgaccc atgggtcccc caggacctcc tgggcctcct
      661 ggacctccac ggagtggtgg tggcatgcct ctgccctcac ggggtggcag accaccccgc
      721 atactgttca ttggaggctt cttccgagca agggagactt taagtttgct cccttgaaaa
      781 tctttcccat caaaccattc cacggcagcc ttggcagtgg gtgggtcttc ataggacact
      841 gtggcatcgc ctttgggctt tcctgtttcc ttgtccaggt agatgtggat catgggttgc
      901 ccagttctct tg
//
