LOCUS       sequence-166837-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DONOR vector for Gateway cloning of EWSR1 g1750a No Stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pDONR221-EWSR1 g1750a No Stop
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtcgta gggccgatct ctgcgctcct
      121 gacggtgctc gcctttatcc atttttccag gtcctccacg tcctcctctt cttcctccca
      181 tctgttccat caaaggtcca gggggccccc cagggccacc tcgtcttcct ccaccaaagc
      241 cacctcggtc catgccccgg ccaccacgga agccacctct gtctccacca cggccacctc
      301 tgaacattcc actgggacca ccacgatcca tgaggccacc tcttcctccc cgcatgccac
      361 cagggccacc tctgccacga tcaccacccg ggggcggaaa gggtggcggg aggaagcctt
      421 caggctttgg ggccttacac tggttgcact ctgttctcca ggcgaagttc tggtttccac
      481 aacccggatt gggacactgc cagtctccag ctcggtgctg gacgtttcct cctccagagg
      541 ggttccctcg ggaaccccgg ggtcctcttg gagggaagcc tcctctatct cctccacggc
      601 ctcccatgcg acccatgggt cccccaggac ctcctgggcc tcctggacct ccacggagtg
      661 gtggtggcat gcctctgccc tcacggggtg gcagaccacc ccgcatactg ttcattggag
      721 gcttcttccg agcaagggag actttaagtt tgctcccttg aaaatctttc ccatcaaacc
      781 attccacggc agccttggca gtgggtgggt cttcatagga cactgtggca tcgcctttgg
      841 gctttcctgt ttccttgtcc aggtagatgt ggatcatggg ttgcccagtt ctcttgttca
      901 tcttaacaac cccacactgc ttaaagaa
//
