LOCUS       sequence-166846-        5785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iDamID plasmid. To express transiently the c-Myc-tagged and 
            optimized E. coli Dam adenine methyltransferase fused to the 
            nuclear-localized mmGFP via flexylinker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Gutierrez-Triana JA, Mateo JL, Ibberson D, Ryu S, Wittbrodt J
  TITLE     iDamIDseq and iDEAR: An improved method and computational pipeline 
            to profile chromatin-binding proteins.
  JOURNAL   Development. 2016 Oct 5. pii: dev.139261.
  PUBMED    27707796
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2016 Oct 5. pii: dev.139261."
COMMENT     Sequence Label: pCS2+ cMyc-oDam_f_GFPoNLS
FEATURES             Location/Qualifiers
     source          1..5785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             98..127
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1061..1774
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1091..1119)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1714..1734
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    1834..1968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1858..1877)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1912..1931
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2081..2101)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2081..2099)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2120..2136)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2120..2136)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2133..2155)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2144..2160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2168..2174,2175..2192,2193..2198))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2213..2234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2351..2368)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2522..3110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2602..2621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3281..4072,4073..4141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3904..3923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4142..4246)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4272..4727)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4409..4430)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4621..4640
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4801..5785
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccaccggt caacatggag caaaagctga tctcggagga
      121 ggacctgggc ggctcaggca agaagaacag ggccttcctg aagtgggctg gcggaaagta
      181 cccactgctg gacgacatca agaggcacct gcccaagggc gagtgcctgg tcgagccttt
      241 cgtgggagcc ggaagcgtgt tcctgaacac cgacttcagc cgctacatcc tggccgacat
      301 caacagcgac ctgatcagcc tgtacaacat cgtgaagatg aggaccgacg agtacgtgca
      361 ggccgctcgc gagctgttcg tgcccgagac aaactgcgcc gaggtgtact accagttcag
      421 agaagagttc aacaagagcc aggacccctt ccgcagggcc gtgctgttcc tgtacctgaa
      481 cagatacgga tacaacggcg cctgcagata caacctgagg ggcgagttca acgtgccctt
      541 cggaagatac aagaagccct acttccccga ggccgagctg taccacttcg ccgagaaggc
      601 ccagaacgca ttcttctact gcgagagcta cgccgacagc atggccaggg ccgacgacgc
      661 ctctgtggtg tactgcgacc ctccctacgc ccctctgagc gccaccgcca acttcaccgc
      721 ctaccacacc aacagcttca ccctggagca gcaggcccac ctggccgaga tcgctgaggg
      781 actggtcgag aggcacatcc ccgtgctgat ctccaaccac gacaccatgc tgaccagaga
      841 gtggtatcag agggccaagc tgcacgtggt caaagtgcgc agatccatca gcagcaacgg
      901 cggaaccaga aagaaggtgg acgagctgct ggccctgtac aagcccggcg tggtgtcccc
      961 tgccaagaag gctagcctga gcggtggagg cggttcaggc ggaggtggct ctggcggtgg
     1021 cggatcggga ggcggtggaa gtgcagccgc gactagtacc atgagtaaag gagaagaact
     1081 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggtacaaatt
     1141 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
     1201 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactc tcacttatgg
     1261 tgttcaatgc ttttcaagat atccagatca tatgaagcgg cacgacttct tcaagagcgc
     1321 catgcctgag ggatacgtgc aggagaggac catcttcttc aaggacgacg ggaactacaa
     1381 gacacgtgct gaagtcaagt ttgagggaga caccctcgtc aacaggatcg agcttaaggg
     1441 aatcgatttc aaggaggacg gaaacatcct cggccacaag ttggaataca actacaactc
     1501 ccacaacgta tacatcatgg ccgacaagca aaagaacggc atcaaagcca acttcaagac
     1561 ccgccacaac atcgaagacg gcggcgtgca actcgctgat cattatcaac aaaatactcc
     1621 aattggcgat gaccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     1681 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacggctgc
     1741 tgggattaca catggcatgg atgaactata caaaccacct cccaagaggc ccaggctgga
     1801 ctaatctaga actatagtga gtcgtattac gtagatccag acatgataag atacattgat
     1861 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     1921 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     1981 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc
     2041 gcggcgccaa tgcattgggc ccggtaccca gcttttgttc cctttagtga gggttaattg
     2101 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2161 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2221 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2281 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2341 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2401 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2461 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2521 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2581 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2641 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2701 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2761 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2821 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2881 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2941 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3001 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3061 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3121 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3181 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3241 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3301 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3361 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3421 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3481 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3541 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3601 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3661 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3721 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3781 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3841 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3901 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3961 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4021 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4081 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4141 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4201 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4261 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4321 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4381 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4441 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4501 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4561 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4621 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4681 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     4741 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagtc
     4801 gaccatagcc aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg
     4861 gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg
     4921 gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacttg
     4981 gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt
     5041 ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg
     5101 gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta
     5161 ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat atatgggttt
     5221 tcctattgac gtagatagcc cctcccaatg ggcggtccca tataccatat atggggcttc
     5281 ctaataccgc ccatagccac tcccccattg acgtcaatgg tctctatata tggtctttcc
     5341 tattgacgtc atatgggcgg tcctattgac gtatatggcg cctcccccat tgacgtcaat
     5401 tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc aataggacca cccaccattg
     5461 acgtcaatgg gatggctcat tgcccattca tatccgttct cacgccccct attgacgtca
     5521 atgacggtaa atggcccact tggcagtaca tcaatatcta ttaatagtaa cttggcaagt
     5581 acattactat tggaaggacg ccagggtaca ttggcagtac tcccattgac gtcaatggcg
     5641 gtaaatggcc cgcgatggct gccaagtaca tccccattga cgtcaatggg gaggggcaat
     5701 gacgcaaatg ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat gggaggtcta
     5761 tataagcaat gctcgtttag ggaac
//
