LOCUS       sequence-166847-         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression and/or retrovirus preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Toyota R, Honjo Y, Imajo R, Satoh A
  TITLE     S-nitrosylation of laforin inhibits its phosphatase activity and is 
            implicated in Lafora disease
  JOURNAL   Sciencematters
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Sciencematters"
COMMENT     Sequence Label: pQCXIP-Laforin Myc C278S
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             584..613
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             629..646
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(669..686)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ttttaatatt gcaggccacc aagccatgca ttattcaaga attctaccaa atatctggct
       61 gggtagctgc cctcgtcagg tggaacatgt aaccatcaaa ctgaagcatg aattggggat
      121 tacagctgta atgaatttcc agactgaatg ggatattgta cagaattcct caggctgtaa
      181 ccgctaccca gagcccatga ctccagacac tatgattaaa ctatataggg aagaaggctt
      241 ggcctacatc tggatgccaa caccagatat gagcaccgaa ggccgagtac agatgctgcc
      301 ccaggcggtg tgcctgctgc atgcgctgct ggagaaggga cacatcgtgt acgtgcactg
      361 caacgctggg gtgggccgct ccaccgcggc tgtcagcggc tggctccagt atgtgatggg
      421 ctggaatctg aggaaggtgc agtatttcct catggccaag aggccggctg tctacattga
      481 cgaagaggcc ttggcccggg cacaagaaga ttttttccag aaatttggga aggttcgttc
      541 ttctgtgtgt agcctgtact cgagtctaga gggcccgcgg ttcgaacaaa aactcatctc
      601 agaagaggat ctgaatatgc ataccggtca tcatcaccat caccattgag tttaaacccg
      661 ctgatcagcc tcgactgtgc cttctagttg cc
//
