LOCUS       sequence-166849-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression and/or retrovirus preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Toyota R, Honjo Y, Imajo R, Satoh A
  TITLE     S-nitrosylation of laforin inhibits its phosphatase activity and is 
            implicated in Lafora disease
  JOURNAL   Sciencematters
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Sciencematters"
COMMENT     Sequence Label: pQCXIP-Laforin Myc 2KA  (K87A, K320A)
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             638..667
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             683..700
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(723..740)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctggattgag gccactggac acaccaatga aatgaagcac acaacagact tctattttaa
       61 tattgcaggc caccaagcca tgcattattc aagaattcta ccaaatatct ggctgggtag
      121 ctgccctcgt caggtggaac atgtaaccat caaactgaag catgaattgg ggattacagc
      181 tgtaatgaat ttccagactg aatgggatat tgtacagaat tcctcaggct gtaaccgcta
      241 cccagagccc atgactccag acactatgat taaactatat agggaagaag gcttggccta
      301 catctggatg ccaacaccag atatgagcac cgaaggccga gtacagatgc tgccccaggc
      361 ggtgtgcctg ctgcatgcgc tgctggagaa gggacacatc gtgtacgtgc actgcaacgc
      421 tggggtgggc cgctccaccg cggctgtctg cggctggctc cagtatgtga tgggctggaa
      481 tctgaggaag gtgcagtatt tcctcatggc caagaggccg gctgtctaca ttgacgaaga
      541 ggccttggcc cgggcacaag aagatttttt ccaggcattt gggaaggttc gttcttctgt
      601 gtgtagcctg tactcgagtc tagagggccc gcggttcgaa caaaaactca tctcagaaga
      661 ggatctgaat atgcataccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
      721 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgcctt
//
