LOCUS       sequence-166853-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression and/or retrovirus preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Toyota R, Honjo Y, Imajo R, Satoh A
  TITLE     S-nitrosylation of laforin inhibits its phosphatase activity and is 
            implicated in Lafora disease
  JOURNAL   Sciencematters
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Sciencematters"
COMMENT     Sequence Label: pQCXIP-Laforin Myc 3CS_new (C266S, C278S, C329S)
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             622..651
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             667..684
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(707..724)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gcacacacca atgaaatgaa gcacacaaca gacttctatt ttaatattgc aggccaccaa
       61 gccatgcatt attcaagaat tctaccaaat atctggctgg gtagctgccc tcgtcaggtg
      121 gaacatgtaa ccatcaaact gaagcatgaa ttggggatta cagctgtaat gaatttccag
      181 actgaatggg atattgtaca gaattcctca ggctgtaacc gctacccaga gcccatgact
      241 ccagacacta tgattaaact atatagggaa gaaggcttgg cctacatctg gatgccaaca
      301 ccagatatga gcaccgaagg ccgagtacag atgctgcccc aggcggtgtg cctgctgcat
      361 gcgctgctgg agaagggaca catcgtgtac gtgcacagca acgctggggt gggccgctcc
      421 accgcggctg tcagcggctg gctccagtat gtgatgggct ggaatctgag gaaggtgcag
      481 tatttcctca tggccaagag gccggctgtc tacattgacg aagaggcctt ggcccgggca
      541 caagaagatt ttttccagaa atttgggaag gttcgttctt ctgtgagtag cctgtactcg
      601 agtctagagg gcccgcggtt cgaacaaaaa ctcatctcag aagaggatct gaatatgcat
      661 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
      721 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
      781 gccactccca ctgtc
//
