LOCUS       sequence-166855-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression and/or retrovirus preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Toyota R, Honjo Y, Imajo R, Satoh A
  TITLE     S-nitrosylation of laforin inhibits its phosphatase activity and is 
            implicated in Lafora disease
  JOURNAL   Sciencematters
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Sciencematters"
COMMENT     Sequence Label: pQCXIP-Laforin Myc R171H
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             635..664
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             680..697
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(720..737)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    726..837
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gattgaggcc actggacaca ccaatgaaat gaagcacaca acagacttct attttaatat
       61 tgcaggccac caagccatgc attattcaag aattctacca aatatctggc tgggtagctg
      121 ccctcatcag gtggaacatg taaccatcaa actgaagcat gaattgggga ttacagctgt
      181 aatgaatttc cagactgaat gggatattgt acagaattcc tcaggctgta accgctaccc
      241 agagcccatg actccagaca ctatgattaa actatatagg gaagaaggct tggcctacat
      301 ctggatgcca acaccagata tgagcaccga aggccgagta cagatgctgc cccaggcggt
      361 gtgcctgctg catgcgctgc tggagaaggg acacatcgtg tacgtgcact gcaacgctgg
      421 ggtgggccgc tccaccgcgg ctgtctgcgg ctggctccag tatgtgatgg gctggaatct
      481 gaggaaggtg cagtatttcc tcatggccaa gaggccggct gtctacattg acgaagaggc
      541 cttggcccgg gcacaagaag attttttcca gaaatttggg aaggttcgtt cttctgtgtg
      601 tagcctgtac tcgagtctag agggcccgcg gttcgaacaa aaactcatct cagaagagga
      661 tctgaatatg cataccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
      721 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
      781 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
      841 ttgtctgagt aggtgtcatt ctattctggg gg
//
