LOCUS       sequence-166857-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression and/or retrovirus preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Toyota R, Honjo Y, Imajo R, Satoh A
  TITLE     S-nitrosylation of laforin inhibits its phosphatase activity and is 
            implicated in Lafora disease
  JOURNAL   Sciencematters
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Sciencematters"
COMMENT     Sequence Label: pQCXIP-Laforin Myc W32G
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             627..656
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             672..689
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(712..729)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ccactggaca caccaatgaa atgaagcaca caacagactt ctattttaat attgcaggcc
       61 accaagccat gcattattca agaattctac caaatatctg gctgggtagc tgccctcgtc
      121 aggtggaaca tgtaaccatc aaactgaagc atgaattggg gattacagct gtaatgaatt
      181 tccagactga atgggatatt gtacagaatt cctcaggctg taaccgctac ccagagccca
      241 tgactccaga cactatgatt aaactatata gggaagaagg cttggcctac atctggatgc
      301 caacaccaga tatgagcacc gaaggccgag tacagatgct gccccaggcg gtgtgcctgc
      361 tgcatgcgct gctggagaag ggacacatcg tgtacgtgca ctgcaacgct ggggtgggcc
      421 gctccaccgc ggctgtctgc ggctggctcc agtatgtgat gggctggaat ctgaggaagg
      481 tgcagtattt cctcatggcc aagaggccgg ctgtctacat tgacgaagag gccttggccc
      541 gggcacaaga agattttttc cagaaatttg ggaaggttcg ttcttctgtg tgtagcctgt
      601 actcgagtct agagggcccg cggttcgaac aaaaactcat ctcagaagag gatctgaata
      661 tgcataccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
      721 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      781 aaggtgccac tcccactgtc ctttcctaat aaaa
//
