LOCUS       sequence-166866-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression and/or retrovirus preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Toyota R, Honjo Y, Imajo R, Satoh A
  TITLE     S-nitrosylation of laforin inhibits its phosphatase activity and is 
            implicated in Lafora disease
  JOURNAL   Sciencematters
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Sciencematters"
COMMENT     Sequence Label: pQCXIP-Laforin Myc 3CS (C250S,C266S,C278S)
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             621..650
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             666..683
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(706..723)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gacacaccaa tgaaatgaag cacacaacag acttctattt taatattgca ggccaccaag
       61 ccatgcatta ttcaagaatt ctaccaaata tctggctggg tagctgccct cgtcaggtgg
      121 aacatgtaac catcaaactg aagcatgaat tggggattac agctgtaatg aatttccaga
      181 ctgaatggga tattgtacag aattcctcag gctgtaaccg ctacccagag cccatgactc
      241 cagacactat gattaaacta tatagggaag aaggcttggc ctacatctgg atgccaacac
      301 cagatatgag caccgaaggc cgagtacaga tgctgcccca ggcggtgagc ctgctgcatg
      361 cgctgctgga gaagggacac atcgtgtacg tgcactccaa cgctggggtg ggccgctcca
      421 ccgcggctgt cagcggctgg ctccagtatg tgatgggctg gaatctgagg aaggtgcagt
      481 atttcctcat ggccaagagg ccggctgtct acattgacga agaggccttg gcccgggcac
      541 aagaagattt tttccagaaa tttgggaagg ttcgttcttc tgtgtgtagc ctgtactcga
      601 gtctagaggg cccgcggttc gaacaaaaac tcatctcaga agaggatctg aatatgcata
      661 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
      721 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctgga
//
