LOCUS       sequence-166870-        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed N-term tagged APEX2 on HMOX1 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Lee SY, Kang MG, Park JS, Lee G, Ting AY, Rhee HW
  TITLE     APEX Fingerprinting Reveals the Subcellular Localization of Proteins
            of Interest.
  JOURNAL   Cell Rep. 2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064.
  PUBMED    27184847
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064."
COMMENT     Sequence Label: pCDNA3_V5-APEX2-HMOX1
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     72..91
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        72..90
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      110..119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             119..160
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             161..907
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 tcgagggtgg aagcatatag cagagctctc tggctaacta gagaacccac tgcttactgg
       61 cttatcgaaa ttaatacgac tcactatagg gagacccaag cttgcggccg ccaccatggg
      121 caagcccatc cccaaccccc tgctgggcct ggacagcacc ggaaagtctt acccaactgt
      181 gagtgctgat taccaggacg ccgttgagaa ggcgaagaag aagctcagag gcttcatcgc
      241 tgagaagaga tgcgctcctc taatgctccg tttggcattc cactctgctg gaacctttga
      301 caagggcacg aagaccggtg gacccttcgg aaccatcaag caccctgccg aactggctca
      361 cagcgctaac aacggtcttg acatcgctgt taggcttttg gagccactca aggcggagtt
      421 ccctattttg agctacgccg atttctacca gttggctggc gttgttgccg ttgaggtcac
      481 gggtggacct aaggttccat tccaccctgg aagagaggac aagcctgagc caccaccaga
      541 gggtcgcttg cccgatccca ctaagggttc tgaccatttg agagatgtgt ttggcaaagc
      601 tatggggctt actgaccaag atatcgttgc tctatctggg ggtcacacta ttggagctgc
      661 acacaaggag cgttctggat ttgagggtcc ctggacctct aatcctctta ttttcgacaa
      721 ctcatacttc acggagttgt tgagtggtga gaaggaaggt ctccttcagc taccttctga
      781 caaggctctt ttgtctgacc ctgtattccg ccctctcgtt gataaatatg cagcggacga
      841 agatgccttc tttgctgatt acgctgaggc tcaccaaaag ctttccgagc ttgggtttgc
      901 tgatgccaag ctagcaatgg agcgtccgca acccgacagc atgccccagg atttgtcaga
      961 ggccctgaag gaggccacca aggaggtgca cacccaggca gagaatgctg agttcatgag
     1021 gaactttcag aagggccagg tgacccgaga cggcttcaag ctggtgatgg cctccctgta
     1081 ccacatctat gtgggcttgg aaggaggaga tg
//
