LOCUS       sequence-166882-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 transposon with EGFP driven by minimal-CAGs promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Clark KJ, Carlson DF, Foster LK, Kong BW, Foster DN, Fahrenkrug SC
  TITLE     Enzymatic engineering of the porcine genome with transposons and 
            recombinases.
  JOURNAL   BMC Biotechnol. 2007 Jul 17;7:42.
  PUBMED    17640337
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-07-17"; volume: "7"; pages: "42"
COMMENT     Sequence Label: pKTol2C-EGFP
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(257..268,269..431)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(451..469)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     812..831
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    830..885
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     931..950
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaacaga
      241 tttaaatggt accgagcaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta
      301 caagtactta gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact
      361 tgagtaaaag taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt
      421 aaaagcaaga aagaaaacta gagtggtctc cctttagtga gggttaattg atatcgctag
      481 cttgaccggt atgcaggtcg agggatctcc ataagagaag agggacagct atgactggga
      541 gtagtcagga gaggaggaaa aatctggcta gtaaaacatg taaggaaaat tttagggatg
      601 ttaaagaaaa aaataacaca aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt
      661 ttctatggaa taaggaatgg acagcagggg gctgtttcat atactgatga cctctttata
      721 gccaaccttt gttcatggca gccagcatat gggcatatgt tgccaaactc taaaccaaat
      781 actcattctg atgttttaaa tgatttgccc tcccatatgt ccttccgagt gagagacaca
      841 aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattagcca gaagtcagat
      901 gctcaagggg cttcatgatg tccccataat ttttggcaga gggaaaaaga tctttacttg
      961 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactcca
//
