LOCUS       sequence-166886-        5181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Source of Tol2 transposase driven by a human ubiquitin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Clark KJ, Carlson DF, Foster LK, Kong BW, Foster DN, Fahrenkrug SC
  TITLE     Enzymatic engineering of the porcine genome with transposons and 
            recombinases.
  JOURNAL   BMC Biotechnol. 2007 Jul 17;7:42.
  PUBMED    17640337
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-07-17"; volume: "7"; pages: "42"
COMMENT     Sequence Label: pKUb-Tol2
FEATURES             Location/Qualifiers
     source          1..5181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..1220
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1057..1076
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1229..3178
                     /codon_start=1
                     /product="transposase of the Tol2 transposon from the 
                     medaka fish Oryzias latipes (Kawakami, 2007)"
                     /label=Tol2 transposase
                     /translation="MEEVCDSSAAASSTVQNQPQDQEHPWPYLREFFSLSGVNKDSFKM
                     KCVLCLPLNKEISAFKSSPSNLRKHIERMHPNYLKNYSKLTAQKRKIGTSTHASSSKQL
                     KVDSVFPVKHVSPVTVNKAILRYIIQGLHPFSTVDLPSFKELISTLQPGISVITRPTLR
                     SKIAEAALIMKQKVTAAMSEVEWIATTTDCWTARRKSFIGVTAHWINPGSLERHSAALA
                     CKRLMGSHTFEVLASAMNDIHSEYEIRDKVVCTTTDSGSNFMKAFRVFGVENNDIETEA
                     RRCESDDTDSEGCGEGSDGVEFQDASRVLDQDDGFEFQLPKHQKCACHLLNLVSSVDAQ
                     KALSNEHYKKLYRSVFGKCQALWNKSSRSALAAEAVESESRLQLLRPNQTRWNSTFMAV
                     DRILQICKEAGEGALRNICTSLEVPMFNPAEMLFLTEWANTMRPVAKVLDILQAETNTQ
                     LGWLLPSVHQLSLKLQRLHHSLRYCDPLVDALQQGIQTRFKHMFEDPEIIAAAILLPKF
                     RTSWTNDETIIKRGMDYIRVHLEPLDHKKELANSSSDDEDFFASLKPTTHEASKELDGY
                     LACVSDTRESLLTFPAICSLSIKTNTPLPASAACERLFSTAGLLFSPKRARLDTNNFEN
                     QLLLKLNLRFYNFE"
     primer_bind     complement(3194..3213)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    3223..3304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3248..3267
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3414..3431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3585..4173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3665..3684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4316..5125)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5039..5058
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5117..5136
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ctgagatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg
       61 cgagcgctgc cacgtcagac gaagggcgca ggagcgtcct gatccttccg cccggacgct
      121 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
      181 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
      241 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
      301 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
      361 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
      421 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
      481 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
      541 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
      601 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
      661 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctgtg gcaccatctg
      721 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
      781 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
      841 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
      901 taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
      961 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     1021 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     1081 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     1141 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     1201 ttttggcttt tttgttagac ggctagccat ggaggaagta tgtgattcat cagcagctgc
     1261 gagcagcaca gtccaaaatc agccacagga tcaagagcac ccgtggccgt atcttcgcga
     1321 attcttttct ttaagtggtg taaataaaga ttcattcaag atgaaatgtg tcctctgtct
     1381 cccgcttaat aaagaaatat cggccttcaa aagttcgcca tcaaacctaa ggaagcatat
     1441 tgagagaatg cacccaaatt acctcaaaaa ctactctaaa ttgacagcac agaagagaaa
     1501 gatcgggacc tccacccatg cttccagcag taagcaactg aaagttgact cagttttccc
     1561 agtcaaacat gtgtctccag tcactgtgaa caaagctata ttaaggtaca tcattcaagg
     1621 acttcatcct ttcagcactg ttgatctgcc atcatttaaa gagctgatta gtacactgca
     1681 gcctggcatt tctgtcatta caaggcctac tttacgctcc aagatagctg aagctgctct
     1741 gatcatgaaa cagaaagtga ctgctgccat gagtgaagtt gaatggattg caaccacaac
     1801 ggattgttgg actgcacgta gaaagtcatt cattggtgta actgctcact ggatcaaccc
     1861 tggaagtctt gaaagacatt ccgctgcact tgcctgcaaa agattaatgg gctctcatac
     1921 ttttgaggta ctggccagtg ccatgaatga tatccactca gagtatgaaa tacgtgacaa
     1981 ggttgtttgc acaaccacag acagtggttc caactttatg aaggctttca gagtttttgg
     2041 tgtggaaaac aatgatatcg agactgaggc aagaaggtgt gaaagtgatg acactgattc
     2101 tgaaggctgt ggtgagggaa gtgatggtgt ggaattccaa gatgcctcac gagtcctgga
     2161 ccaagacgat ggcttcgaat tccagctacc aaaacatcaa aagtgtgcct gtcacttact
     2221 taacctagtc tcaagcgttg atgcccaaaa agctctctca aatgagcact acaagaaact
     2281 ctacagatct gtctttggca aatgccaagc tttatggaat aaaagcagcc gatcggctct
     2341 agcagctgaa gctgttgaat cagaaagccg gcttcagctt ttaaggccaa accaaacgcg
     2401 gtggaattca acttttatgg ctgttgacag aattcttcaa atttgcaaag aagcaggaga
     2461 aggcgcactt cggaatatat gcacctctct tgaggttcca atgtttaatc cagcagaaat
     2521 gctgttcttg acagagtggg ccaacacaat gcgtccagtt gcaaaagtac tcgacatctt
     2581 gcaagcggaa acgaatacac agctggggtg gctgctgcct agtgtccatc agttaagctt
     2641 gaaacttcag cgactccacc attctctcag gtactgtgac ccacttgtgg atgccctaca
     2701 acaaggaatc caaacacgat tcaagcatat gtttgaagat cctgagatca tagcagctgc
     2761 catccttctc cctaaatttc ggacctcttg gacaaatgat gaaaccatca taaaacgagg
     2821 catggactac atcagagtgc atctggagcc tttggaccac aagaaggaat tggccaacag
     2881 ttcatctgat gatgaagatt ttttcgcttc tttgaaaccg acaacacatg aagccagcaa
     2941 agagttggat ggatatctgg cctgtgtttc agacaccagg gagtctctgc tcacgtttcc
     3001 tgctatttgc agcctctcta tcaagactaa tacacctctt cccgcatcgg ctgcctgtga
     3061 gaggcttttc agcactgcag gattgctttt cagccccaaa agagctaggc ttgacactaa
     3121 caattttgag aatcagcttc tactgaagtt aaatctgagg ttttacaact ttgagtagga
     3181 tccagaattc attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     3241 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     3301 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     3361 tttttaaagc aagtaaaacc tctacaaatg tggtcattca gctgttccgc ttcctcgctc
     3421 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3481 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3541 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3601 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3661 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3721 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaaccgtggc gctttctcat
     3781 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3841 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3901 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3961 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4021 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4081 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4141 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4201 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4261 aggatcttca cctagatcct ttttgccagt gttacaacca attaaccaat tctgattaga
     4321 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     4381 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     4441 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     4501 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     4561 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     4621 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     4681 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     4741 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     4801 ctaatacctg gaatgctgtt tttccgggga tcgcagtggt gagtaaccat gcatcatcag
     4861 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     4921 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     4981 ctggcgcatc gggcttccca tacaagcgat agattgtcgc acctgattgc ccgacattat
     5041 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg
     5101 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     5161 gttttattgt tcatgatgca g
//
