LOCUS       sequence-166897-         467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression, GST-fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Toyota R, Honjo Y, Imajo R, Satoh A
  TITLE     S-nitrosylation of laforin inhibits its phosphatase activity and is 
            implicated in Lafora disease
  JOURNAL   Sciencematters
REFERENCE   2  (bases 1 to 467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Sciencematters"
COMMENT     Sequence Label: pGEX6P1-GST-Laforin 2CS_new (C278S, C329S)
FEATURES             Location/Qualifiers
     source          1..467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(34..63)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atggtgatgg tgatgatgac cggnatgcat attcagatcc tcttctgaga tgagtttttg
       61 ttcgaaccgc gggccctcta gactcgagta caggctactc acagaagaac gaaccttccc
      121 aaatttctgg aaaaaatctt cttgtgcccg ggccaaggcc tcttcgtcaa tgtagacagc
      181 cggcctcttg gccatgagga aatactgcac cttcctcaga ttccagccca tcacatactg
      241 gagccagccg ctgacagccg cggtggagcg gcccacccca gcgttgcagt gcacgtacac
      301 gatgtgtccc ttctccagca gcgcatgcag caggcacacc gcctggggca gcatctgtac
      361 tcggccttcg gtgctcatat ctggtgttgg catccagatg taggccaagc cttcttccct
      421 atatagttta atcatagtgt ctggagtcat gggctctggg tagcggt
//
