LOCUS       sequence-166899-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of doxycycline-inducible dominant negative 
            Rac1 and co-expression of Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   MacKay JL, Kumar S
  TITLE     Simultaneous and independent tuning of RhoA and Rac1 activity with 
            orthogonally inducible promoters.
  JOURNAL   Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d.
  PUBMED    25044255
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Integr Biol (Camb)"; date: "2014-09"; volume: "6"; issue: "9"; 
            pages: "885-94"
COMMENT     Sequence Label: pSLIK DN Rac1
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    77..665
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(130..150)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(548..559)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(668..684)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..685)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtaaga attgttgttg tttagtccct cccaattcga
       61 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
      121 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
      181 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
      241 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
      301 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
      361 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
      421 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
      481 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
      541 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
      601 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
      661 cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca atcacaagta
      721 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
      781 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
      841 atcttagcca ctttttaaaa gaaaaggggg gactggaagg g
//
