LOCUS       sequence-166906-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GA-inducible KRAB-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE
            PGK...
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             465..650
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 ttctgctgaa tcctatgttt ggtgggaatg agagaacgga gtctgagaaa agtttggatg
       61 ggaaatactt tgtgacggtt agagaccgcg attggtactg gaaagcgttt ttacccgagg
      121 gagaagatag agagcatcca gcgtgtaatc cgtttagccc gagagggaaa agcttagaag
      181 gagtgagttt ccccaagagt cttgtggttg tcgcgggttt ggatttgatt agagattggc
      241 agttggcata cgcggaaggg ctcaagaaag cgggtcaaga ggttaagctt atgcatttag
      301 agaaagcaac tgttgggttt tacctcttgc ctaataacaa tcatttccat aatgttatgg
      361 atgagatttc ggcgtttgta aacgcggaat gtgaattctc aagcgctgca ggtactagtg
      421 gcggtggcgg agggatggat gctaagtcac taactgcctg gtcccggaca ctggtgacct
      481 tcaaggatgt atttgtggac ttcaccaggg aggagtggaa gctgctggac actgctcagc
      541 agatcgtgta cagaaatgtg atgctggaga actataagaa cctggtttcc ttgggttatc
      601 agcttactaa gccagatgtg atcctccggt tggagaaggg agaagagccc tagatttaaa
      661 taattcgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
      721 gccggtgtgc gtttgtctat atgttatttt ccacc
//
