LOCUS       sequence-166910-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GA-inducible VPR-Sa dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE
            PGK-G...
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             420..569
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             576..596
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttctgctgaa tcctatgttt ggtgggaatg agagaacgga gtctgagaaa agtttggatg
       61 ggaaatactt tgtgacggtt agagaccgcg attggtactg gaaagcgttt ttacccgagg
      121 gagaagatag agagcatcca gcgtgtaatc cgtttagccc gagagggaaa agcttagaag
      181 gagtgagttt ccccaagagt cttgtggttg tcgcgggttt ggatttgatt agagattggc
      241 agttggcata cgcggaaggg ctcaagaaag cgggtcaaga ggttaagctt atgcatttag
      301 agaaagcaac tgttgggttt tacctcttgc ctaataacaa tcatttccat aatgttatgg
      361 atgagatttc ggcgtttgta aacgcggaat gtgaattctc aagcgctgca ggtactagtg
      421 atgctttaga cgattttgac ttagatatgc ttggttcaga cgcgttagac gacttcgacc
      481 tagacatgtt aggctcagat gcattggacg acttcgattt agatatgttg ggctccgatg
      541 ccctagatga ctttgatcta gatatgctag gtagtcccaa aaagaagagg aaagtgggat
      601 cccagtatct gcccgacaca gatgatagac accgaatcga agagaaacgc aagcgaacgt
      661 atgaaacctt caaatcgatc atgaagaaat cgcccttctc gggtccgacc gatcccaggc
      721 ccccaccgag aaggattgcg gtcccgtccc gctcgtcggc cagcgtgccg aagcctgcgc
      781 cgcagcccta ccccttcacg tcgagcctga gcacaatcaa ttatgacgag ttcccgacga
      841 tggtgttccc ctcgggacaa atctcacaag cctcggcgct cgcacca
//
