LOCUS       sequence-166918-        4815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KIF2B , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_KIF2B_p.Q430H
FEATURES             Location/Qualifiers
     source          1..4815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2215..2314)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2331..2350)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2332..2350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2355..2371)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2355..2371)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2802..3593
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3686..4274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4175..4194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4428..4445
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4604..4631)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4723..4809)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggccagc cagttctgcc tccctgaatc cccatgtctc tcgcccctga
      241 aacccttgaa gccacatttc ggagacatcc aagagggcat ctacgtggcg atccagcgca
      301 gtgacaagcg gatccacctc gctgtggtca cggagatcaa cagagaaaac tattgggtca
      361 cggtagagtg ggtggagaaa gcagtcaaaa aaggcaagaa gattgacctg gagaccatac
      421 tcctgctgaa tccagctctg gactctgctg aacaccccat gccgcccccg cccttatccc
      481 ccttggctct ggcgccctct tcggccatca gggaccagcg taccgccacg aaatgggttg
      541 cgatgatccc ccagaaaaac caaacagcct caggggacag cctggatgtg agggtcccca
      601 gcaaaccttg tctgatgaag cagaaaaagt ctccctgcct ctgggaaatc cagaaactgc
      661 aggagcagcg ggaaaagcgc aggcggctgc agcaggagat ccgagctaga cgcgccctcg
      721 atgtcaatac cagaaacccc aactacgaaa tcatgcacat gatcgaagag tatcgcaggc
      781 acctggacag cagcaagatc tcagtcctgg agcccccgca agaacatcgc atctgcgtct
      841 gcgtgaggaa gcggcctctc aaccagcgag agacaacctt gaaggacctg gatatcatca
      901 ccgtcccctc ggacaatgtg gttatggtgc atgagtccaa gcaaaaggtg gacctcactc
      961 gctacctgca gaaccagacc ttctgcttcg accatgcctt cgatgacaaa gcctccaacg
     1021 agttggtgta ccagttcacc gcccagccac tggtggagtc catcttccgc aagggcatgg
     1081 ccacctgctt tgcctatggg cagacgggaa gtgggaagac gtacaccatg ggtggagact
     1141 tttcaggaac ggcccaagat tgttctaagg gcatttatgc tttggtggca caggatgtct
     1201 ttctcctgct cagaaactcc acatatgaga agctggacct caaagtctat gggacatttt
     1261 ttgagattta tgggggcaag gtgtatgatt tgttgaactg gaagaagaag ctgcaagtcc
     1321 ttgaggatgg caatcagcaa atccaagtgg tcgggctgca ggagaaagag gtgtgttgtg
     1381 tggaggaagt gctgaacctg gtggaaatag ggaatagctg tcggacttcc aggcaaacat
     1441 ctgtcaacgc tcactcatcc aggagccatg cagtgttcca catcatcctg aagtcaggag
     1501 ggataatgca tggcaagttt tccctcgttg atttagctgg gaatgaaaga ggagcagata
     1561 caaccaaggc cagccggaaa aggcagctgg aaggggcaga gattaacaag agtcttctag
     1621 ccctcaaaga atgtattctg gctttgggtc agaacaagcc tcacacccca ttcagagcca
     1681 gcaaactcac actggtgctc cgggactcct ttataggcca gaactcctcc acttgcatga
     1741 ttgctaccat ctctccgggg atgacctctt gtgaaaacac tctcaacact ttaagatatg
     1801 caaacagagt aaaaaaatta aatgtagatg taaggcccta ccatcgtggc cactatccga
     1861 ttggacatga ggcaccaagg atgttaaaaa gtcacatcgg aaattcagaa atgtcccttc
     1921 agagggatga atttattaaa ataccttatg tacagagtga ggagcagaaa gagattgaag
     1981 aggttgaaac attacccact ctgttaggga aggataccac aatttcaggg aagggatcta
     2041 gccaatggtt ggaaaacatc caggagagag ctggtggagt acaccatgat attgattttt
     2101 gcattgcccg gtctttgtcc attttggagc agaaaattga tgctctgacc gagatccaaa
     2161 agaaactgaa attattacta gctgacctcc acgtgaagag caaggtagag tgattgccaa
     2221 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     2281 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     2341 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2401 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     2461 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     2521 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     2581 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     2641 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     2701 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2761 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     2821 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2881 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2941 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     3001 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     3061 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     3121 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     3181 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     3241 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     3301 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     3361 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     3421 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     3481 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3541 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     3601 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     3661 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3721 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3781 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3841 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3901 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3961 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4021 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4081 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4141 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4201 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4261 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4321 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4381 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4441 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4501 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4561 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     4621 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     4681 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4741 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4801 ttttatttga tgcct
//
