LOCUS       sequence-166921-        4977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTCF , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTCF_p.R377H
FEATURES             Location/Qualifiers
     source          1..4977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2377..2476)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2493..2512)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2494..2512)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2517..2533)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2517..2533)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2964..3755
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3848..4436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4337..4356
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4590..4607
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4766..4793)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4885..4971)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggaaggt gatgcagtcg aagccattgt ggaggagtcc gaaactttta
      241 ttaaaggaaa ggagagaaag acttaccaga gacgccggga agggggccag gaagaagatg
      301 cctgccactt accccagaac cagacggatg ggggtgaggt ggtccaggat gtcaacagca
      361 gtgtacagat ggtgatgatg gaacagctgg accccaccct tcttcagatg aagactgaag
      421 taatggaggg cacagtggct ccagaagcag aggctgctgt ggacgatacc cagattataa
      481 ctttacaggt tgtaaatatg gaggaacagc ccataaacat aggagaactt cagcttgttc
      541 aagtacctgt tcctgtgact gtacctgttg ctaccacttc agtagaagaa cttcaggggg
      601 cttatgaaaa tgaagtgtct aaagagggcc ttgcggaaag tgaacccatg atatgccaca
      661 ccctaccttt gcctgaaggg tttcaggtgg ttaaagtggg ggccaatgga gaggtggaga
      721 cactagaaca aggggaactt ccaccccagg aagatcctag ttggcaaaaa gacccagact
      781 atcagccacc agccaaaaaa acaaagaaaa ccaaaaagag caaactgcgt tatacagagg
      841 agggcaaaga tgtagatgtg tctgtctacg attttgagga agaacagcag gagggtctgc
      901 tatcagaggt taatgcagag aaagtggttg gtaatatgaa gcctccaaag ccaacaaaaa
      961 ttaaaaagaa aggtgtaaag aagacattcc agtgtgagct ttgcagttac acgtgtccac
     1021 ggcgttcaaa tttggatcgt cacatgaaaa gccacactga tgagagacca cacaagtgcc
     1081 atctctgtgg cagggcattc agaacagtca ccctcctgag gaatcacctt aacacacaca
     1141 caggtactcg tcctcacaag tgcccagact gcgacatggc ctttgtgacc agtggagaat
     1201 tggttcggca tcgtcgttac aaacacaccc acgagaagcc attcaagtgt tccatgtgcg
     1261 attacgccag tgtagaagtc agcaaattaa aacgtcacat tcgctctcat actggagagc
     1321 atccgtttca gtgcagtttg tgcagttatg ccagcaggga cacatacaag ctgaaaaggc
     1381 acatgagaac ccattcaggg gaaaagcctt atgaatgtta tatttgtcat gctcggttta
     1441 cccaaagtgg taccatgaag atgcacattt tacagaagca cacagaaaat gtggccaaat
     1501 ttcactgtcc ccactgtgac acagtcatag cccgaaaaag tgatttgggt gtccacttgc
     1561 gaaagcagca ttcctatatt gagcaaggca agaaatgccg ttactgtgat gctgtgtttc
     1621 atgagcgcta tgccctcatc cagcatcaga agtcacacaa gaatgagaag cgctttaagt
     1681 gtgaccagtg tgattacgct tgtagacagg agaggcacat gatcatgcac aagcgcaccc
     1741 acaccgggga gaagccttac gcctgcagcc actgcgataa gaccttccgc cagaagcagc
     1801 ttctcgacat gcacttcaag cgctatcacg accccaactt cgtccctgcg gcttttgtct
     1861 gttctaagtg tgggaaaaca tttacacgtc ggaataccat ggcaagacat gctgataatt
     1921 gtgctggccc agatggcgta gagggggaaa atggaggaga aacgaagaag agtaaacgtg
     1981 gaagaaaaag aaagatgcgc tctaagaaag aagattcctc tgacagtgaa aatgctgaac
     2041 cagatctgga cgacaatgag gatgaggagg agcctgccgt agaaattgaa cctgagccag
     2101 agcctcagcc tgtgacccca gccccaccac ccgccaagaa gcggagagga cgaccccctg
     2161 gcagaaccaa ccagcccaaa cagaaccagc caacagctat cattcaggtt gaagaccaga
     2221 atacaggtgc aattgagaac attatagttg aagtaaaaaa agagccagat gctgagcccg
     2281 cagagggaga ggaagaggag gcccagccag ctgccacaga tgcccccaac ggagacctca
     2341 cgcccgagat gatcctcagc atgatggacc ggtgattgcc aactttcttg tacaaagttg
     2401 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     2461 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     2521 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     2581 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
     2641 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     2701 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     2761 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2821 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2881 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2941 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     3001 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3061 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3121 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3181 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3241 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3301 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3361 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3421 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3481 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     3541 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     3601 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     3661 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     3721 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     3781 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3841 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3901 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3961 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4021 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4081 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4141 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4201 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4261 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4321 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4381 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4441 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4501 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4561 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4621 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4681 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     4741 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4801 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4861 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4921 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcct
//
