LOCUS       sequence-166924-        4665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KEAP1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_KEAP1_p.P318L
FEATURES             Location/Qualifiers
     source          1..4665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2065..2164)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2181..2200)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2182..2200)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2205..2221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2205..2221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2652..3443
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3536..4124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4025..4044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4278..4295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4454..4481)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4573..4659)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgcagcca gatcccaggc ctagcggggc tggggcctgc tgccgattcc
      241 tgcccctgca gtcacagtgc cctgaggggg caggggacgc ggtgatgtac gcctccactg
      301 agtgcaaggc ggaggtgacg ccctcccagc atggcaaccg caccttcagc tacaccctgg
      361 aggatcatac caagcaggcc tttggcatca tgaacgagct gcggctcagc cagcagctgt
      421 gtgacgtcac actgcaggtc aagtaccagg atgcaccggc cgcccagttc atggcccaca
      481 aggtggtgct ggcctcatcc agccctgtct tcaaggccat gttcaccaac gggctgcggg
      541 agcagggcat ggaggtggtg tccattgagg gtatccaccc caaggtcatg gagcgcctca
      601 ttgaattcgc ctacacggcc tccatctcca tgggcgagaa gtgtgtcctc cacgtcatga
      661 acggtgctgt catgtaccag atcgacagcg ttgtccgtgc ctgcagtgac ttcctggtgc
      721 agcagctgga ccccagcaat gccatcggca tcgccaactt cgctgagcag attggctgtg
      781 tggagttgca ccagcgtgcc cgggagtaca tctacatgca ttttggggag gtggccaagc
      841 aagaggagtt cttcaacctg tcccactgcc aactggtgac cctcatcagc cgggacgacc
      901 tgaacgtgcg ctgcgagtcc gaggtcttcc acgcctgcat caactgggtc aagtacgact
      961 gcgaacagcg acggttctac gtccaggcgc tgctgcgggc cgtgcgctgc cactcgttga
     1021 cgccgaactt cctgcagatg cagctgcaga agtgcgagat cctgcagtcc gactcccgct
     1081 gcaaggacta cctggtcaag atcttcgagg agctcaccct gcacaagccc acgcaggtga
     1141 tgctctgccg ggcgcccaag gtgggccgcc tgatctacac cgcgggcggc tacttccgac
     1201 agtcgctcag ctacctggag gcttacaacc ccagtgacgg cacctggctc cggttggcgg
     1261 acctgcaggt gccgcggagc ggcctggccg gctgcgtggt gggcgggctg ttgtacgccg
     1321 tgggcggcag gaacaactcg cccgacggca acaccgactc cagcgccctg gactgttaca
     1381 accccatgac caatcagtgg tcgccctgcg cccccatgag cgtgccccgt aaccgcatcg
     1441 gggtgggggt catcgatggc cacatctatg ccgtcggcgg ctcccacggc tgcatccacc
     1501 acaacagtgt ggagaggtat gagccagagc gggatgagtg gcacttggtg gccccaatgc
     1561 tgacacgaag gatcggggtg ggcgtggctg tcctcaatcg tctcctttat gccgtggggg
     1621 gctttgacgg gacaaaccgc cttaattcag ctgagtgtta ctacccagag aggaacgagt
     1681 ggcgaatgat cacagcaatg aacaccatcc gaagcggggc aggcgtctgc gtcctgcaca
     1741 actgtatcta tgctgctggg ggctatgatg gtcaggacca gctgaacagc gtggagcgct
     1801 acgatgtgga aacagagacg tggactttcg tagcccccat gaagcaccgg cgaagtgccc
     1861 tggggatcac tgtccaccag gggagaatct acgtccttgg aggctatgat ggtcacacgt
     1921 tcctggacag tgtggagtgt tacgacccag atacagacac ctggagcgag gtgacccgaa
     1981 tgacatcggg ccggagtggg gtgggcgtgg ctgtcaccat ggagccctgc cggaagcaga
     2041 ttgaccagca gaactgtacc agttgcccaa ctttcttgta caaagttggc attataagaa
     2101 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     2161 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     2221 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2281 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     2341 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     2401 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2461 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2521 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2581 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2641 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     2701 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     2761 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     2821 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     2881 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     2941 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     3001 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     3061 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     3121 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     3181 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     3241 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     3301 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     3361 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     3421 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     3481 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3541 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3601 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3661 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3721 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3781 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3841 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3901 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3961 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4021 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4081 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4141 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4201 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4261 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4321 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4381 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     4441 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     4501 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     4561 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4621 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcct
//
