LOCUS       sequence-166926-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR 
            gate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-m...
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(158..727)
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat atggtggaaa
       61 ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca gtaacgttag
      121 gggggggggg gggagagggg cgaattgttt aaactcaaaa cagagatgtg tcgaagatgg
      181 acagtcctgt gctgatatgc atggcatgca agaggcactc gtcgttcagg aaggtatcca
      241 gaatctcgtt caattccggg gtcaggggtg agtccaggtt cagatcctcg gtcatggact
      301 caagtgtggt tgtcagctca tccagatggc cccttggggg cggatgggaa aggtccattt
      361 ggccacagat tgcagcctct tccttctggg gaatcacagt atcggccatc tcccgaaggg
      421 ctttgacagc ctggctcgtc tcttcatcgg gatcctccaa caggtgactg gcctcgggag
      481 tcactgcggg cgctggatcc agtggctgag ggactggtgc cggggtcagt gacccgactg
      541 gctcatgtac tggaccggtt ggtgttggtg cgaggctggc ggggagtggg cggttggccc
      601 atggacttcc tggaggatga aatggccgga ttcgttttgg ctggcacacc tcgcggccct
      661 caaacacgtc actaatagcg gagccggcct caggcttcgg caaaaacatc ccttccctgg
      721 aatcccggct gccgctgcca gagccgctgg aaatctgtga cagcaaggct gagaaatcca
      781 tatcggcgat ggaggagaaa tcctcgtc
//
