LOCUS       sequence-166930-         406 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for 
            diametric regulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-m...
FEATURES             Location/Qualifiers
     source          1..406
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(162..347)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 gtcaagaaga cagggccagg tttccgggcc ctcacattgc caaaagacgg caatatggtg
       61 gaaaataaca tatagacaaa cgcacaccgg ccttattcca agcggcttcg gccagtaacg
      121 ttaggggggg gggggggaga ggggcgaatt gtttaaacct agggctcttc tcccttctcc
      181 aaccggagga tcacatctgg cttagtaagc tgataaccca aggaaaccag gttcttatag
      241 ttctccagca tcacatttct gtacacgatc tgctgagcag tgtccagcag cttccactcc
      301 tccctggtga agtccacaaa tacatccttg aaggtcacca gtgtccggga cccggcagtt
      361 agtgacttag catccatccc tccgccaccg ccactagtac ctgcag
//
