LOCUS       sequence-166933-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd-generation lentiviral destination vector for gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: pEpic_Lite
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..68
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     52..68
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    88..211
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(252..557)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(354..373)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aattcaaaat tttatcgatc acgagactag cctcgagaag cttgattcga gcaggaaaca
       61 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgaa cgagaaacgt
      121 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
      181 actgtaaaac acaacatatc cagtcactat ggtcgacctg cagactggct gtgtataagg
      241 gagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg
      301 cggtggctga gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg
      361 gtggtcatca tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacggggg
      421 actttatctg acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg
      481 tcaataatat cactctgtac atccacaaac agacgataac ggctctctct tttataggtg
      541 taaaccttaa actgcatttc accagcccct gttctcgtcg gcaaaggagc cgttcatttc
      601 aataaaccgg gcgacctcag ccatcccttc ctgattttcc gctttccagc gttcggcacg
      661 cagacgacgg gcttcattct gcatggttgt gcttaccgaa ccggagatat tgacatcata
      721 tatgccttga gcaactgata gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata
      781 gcatacctct ttttgacata cttcgggtat acatatcagt atatattctt ataccgca
//
