LOCUS       sequence-166942-        4983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BAP1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BAP1_p.N299S
FEATURES             Location/Qualifiers
     source          1..4983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1380..1391
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(2383..2482)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2499..2518)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2500..2518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2523..2539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2523..2539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2970..3761
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3854..4442
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4343..4362
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4596..4613
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4772..4799)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4891..4977)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgaataag ggctggctgg agctggagag cgacccaggc ctcttcaccc
      241 tgctcgtgga agatttcggt gtcaaggggg tgcaagtgga ggagatctac gaccttcaga
      301 gcaaatgtca gggccctgta tatggattta tcttcctgtt caaatggatc gaagagcgcc
      361 ggtcccggcg aaaggtctct accttggtgg atgatacgtc cgtgattgat gatgatattg
      421 tgaataacat gttctttgcc caccagctga tacccaactc ttgtgcaact catgccttgc
      481 tgagcgtgct cctgaactgc agcagcgtgg acctgggacc caccctgagt cgcatgaagg
      541 acttcaccaa gggttttagc cctgagagca aaggatatgc gattggcaat gccccggagt
      601 tggccaaggc ccataatagc catgccaggc ccgagccacg ccacctccct gagaagcaga
      661 atggccttag tgcagtgcgg accatggagg cgttccactt tgtcagctat gtgcctatca
      721 caggccggct ctttgagctg gatgggctga aggtctaccc cattgaccat gggccctggg
      781 gggaggacga ggagtggaca gacaaggccc ggcgggtcat catggagcgt atcggcctcg
      841 ccactgcagg ggagccctac cacgacatcc gcttcaacct gatggcagtg gtgcccgacc
      901 gcaggatcaa gtatgaggcc aggctgcatg tgctgaaggt gaaccgtcag acagtactag
      961 aggctctgca gcagctgata agagtaacac agccagagct gattcagacc cacaagtctc
     1021 aagagtcaca gctgcctgag gagtccaagt cagccagcaa caagtccccg ctggtgctgg
     1081 aagcaagcag ggcccctgca gcctctgagg gcaaccacac agatggtgca gaggaggcgg
     1141 ctggttcatg cgcacaagcc ccatcccaca gccctcccaa caaacccaag ctagtggtga
     1201 agcctccagg cagcagcctc aatggggttc accccaaccc cactcccatt gtccagcggc
     1261 tgccggcctt tctagacaat cacaattatg ccaagtcccc catgcaggag gaagaagacc
     1321 tggcggcagg tgtgggccgc agccgagttc cagtccgccc accccagcag tactcagatg
     1381 atgaggatga ctatgaggat gacgaggagg atgacgtgca gaacaccaac tctgccctta
     1441 ggtataaggg gaagggaaca gggaagccag gggcattgag cggttctgct gatgggcaac
     1501 tgtcagtgct gcagcccaac accatcaacg tcttggctga gaagctcaaa gagtcccaga
     1561 aggacctctc aattcctctg tccatcaaga ctagcagcgg ggctgggagt ccggctgtgg
     1621 cagtgcccac acactcgcag ccctcaccca cccccagcaa tgagagtaca gacacggcct
     1681 ctgagatcgg cagtgctttc aactcgccac tgcgctcgcc tatccgctca gccaacccga
     1741 cgcggccctc cagccctgtc acctcccaca tctccaaggt gctttttgga gaggatgaca
     1801 gcctgctgcg tgttgactgc atacgctaca accgtgctgt ccgtgatctg ggtcctgtca
     1861 tcagcacagg cctgctgcac ctggctgagg atggggtgct gagtcccctg gcgctgacag
     1921 agggtgggaa gggttcctcg ccctccatca gaccaatcca aggcagccag gggtccagca
     1981 gcccagtgga gaaggaggtc gtggaagcca cggacagcag agagaagacg gggatggtga
     2041 ggcctggcga gcccttgagt ggggagaaat actcacccaa ggagctgctg gcactgctga
     2101 agtgtgtgga ggctgagatt gcaaactatg aggcgtgcct caaggaggag gtagagaaga
     2161 ggaagaagtt caagattgat gaccagagaa ggacccacaa ctacgatgag ttcatctgca
     2221 cctttatctc catgctggct caggaaggca tgctggccaa cctagtggag cagaacatct
     2281 ccgtgcggcg gcgccaaggg gtcagcatcg gccggctcca caagcagcgg aagcctgacc
     2341 ggcggaaacg ctctcgcccc tacaaggcca agcgccagtg attgccaact ttcttgtaca
     2401 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     2461 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     2521 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     2581 cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga
     2641 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     2701 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     2761 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     2821 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     2881 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     2941 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     3001 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     3061 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     3121 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     3181 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     3241 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     3301 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     3361 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     3421 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     3481 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     3541 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     3601 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     3661 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     3721 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     3781 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     3841 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3901 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3961 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4021 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4081 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4141 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4201 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4261 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4321 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4381 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4441 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4501 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4561 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4621 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4681 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     4741 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     4801 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     4861 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     4921 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     4981 cct
//
