LOCUS       sequence-166945-        4500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TRAF3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TRAF3_p.N312S
FEATURES             Location/Qualifiers
     source          1..4500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1900..1999)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2016..2035)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2017..2035)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2040..2056)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2040..2056)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2487..3278
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3371..3959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3860..3879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4113..4130
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4289..4316)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4408..4494)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggagtcg agtagaaaga tggactctcc tggcgcgctg cagactaacc
      241 cgccgctaaa gctgcacact gaccgcagtg ctgggacgcc agtttttgtc cctgaacaag
      301 gaggttacaa ggaaaagttt gtgaagaccg tggaggacaa gtacaagtgt gagaagtgcc
      361 acctggtgct gtgcagcccg aagcagaccg agtgtgggca ccgcttctgc gagagctgca
      421 tggcggccct gctgagctct tcaagtccaa aatgtacagc gtgtcaagag agcatcgtta
      481 aagataaggt gtttaaggat aattgctgca agagagaaat tctggctctt cagatctatt
      541 gtcggaatga aagcagaggt tgtgcagagc agttaatgct gggacatctg ctggtgcatt
      601 taaaaaatga ttgccatttt gaagaacttc catgtgtgcg tcctgactgc aaagaaaagg
      661 tcttgaggaa agacctgcga gaccacgtgg agaaggcgtg taaataccgg gaagccacat
      721 gcagccactg caagagtcag gttccgatga tcgcgctgca gaaacacgaa gacaccgact
      781 gtccctgcgt ggtggtgtcc tgccctcaca agtgcagcgt ccagactctc ctgaggagcg
      841 agttgagtgc acacttgtca gagtgtgtca atgcccccag cacctgtagt tttaagcgct
      901 atggctgcgt ttttcagggg acaaaccagc agatcaaggc ccacgaggcc agctccgccg
      961 tgcagcacgt caacctgctg aaggagtgga gcaactcgct cgaaaagaag gtttccttgt
     1021 tgcagaatga aagtgtagaa aaaaacaaga gcatacaaag tttgcacaat cagatatgta
     1081 gctttgaaat tgaaattgag agacaaaagg aaatgcttcg aaatagtgaa tccaaaatcc
     1141 ttcatttaca gcgagtgata gacagccaag cagagaaact gaaggagctt gacaaggaga
     1201 tccggccctt ccggcagaac tgggaggaag cagacagcat gaagagcagc gtggagtccc
     1261 tccagaaccg cgtgaccgag ctggagagcg tggacaagag cgcggggcaa gtggctcgga
     1321 acacaggcct gctggagtcc cagctgagcc ggcatgacca gatgctgagt gtgcacgaca
     1381 tccgcctagc cgacatggac ctgcgcttcc aggtcctgga gaccgccagc tacaatggag
     1441 tgctcatctg gaagattcgc gactacaagc ggcggaagca ggaggccgtc atggggaaga
     1501 ccctgtccct ttacagccag cctttctaca ctggttactt tggctataag atgtgtgcca
     1561 gggtctacct gaacggggac gggatgggga aggggacgca cttgtcgctg ttttttgtca
     1621 tcatgcgtgg agaatatgat gccctgcttc cttggccgtt taagcagaaa gtgacactca
     1681 tgctgatgga tcaggggtcc tctcgacgtc atttgggaga tgcattcaag cccgacccca
     1741 acagcagcag cttcaagaag cccactggag agatgaatat cgcctctggc tgcccagtct
     1801 ttgtggccca aactgttcta gaaaatggga catatattaa agatgataca atttttatta
     1861 aagtcatagt ggatacttcg gatctgcccg atccctgatt gccaactttc ttgtacaaag
     1921 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1981 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     2041 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     2101 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
     2161 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     2221 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     2281 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     2341 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2401 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2461 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     2521 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2581 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     2641 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2701 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2761 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2821 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2881 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2941 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     3001 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     3061 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     3121 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     3181 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     3241 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     3301 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3361 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3421 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3481 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3541 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3601 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3661 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3721 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3781 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3841 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3901 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3961 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4021 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4081 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4141 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4201 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     4261 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4321 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4381 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4441 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
//
