LOCUS       sequence-166952-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase under the control of ATP5O 5'UTR followed by a 
            stem-loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Gandin V, Masvidal L, Hulea L, Gravel SP, Cargnello M, McLaughlan S,
            Cai Y, Balanathan P, Morita M, Rajakumar A, Furic L, Pollak M, Porco
            JA Jr, St-Pierre J, Pelletier J, Larsson O, Topisirovic I
  TITLE     nanoCAGE reveals 5' UTR features that define specific modes of 
            translation of functionally related MTOR-sensitive mRNAs.
  JOURNAL   Genome Res. 2016 May;26(5):636-48. doi: 10.1101/gr.197566.115. Epub 
            2016 Mar 16.
  PUBMED    26984228
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.197566.115"; journalName: "Genome Res"; date: "2016-05";
            volume: "26"; issue: "5"; pages: "636-48"
COMMENT     Sequence Label: pCDNA3 ATP5O(5'UTR)-SL-FF
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..12
                     /estimated_length=11
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(201..219)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     gap             941..955
                     /estimated_length=15
     gap             969..980
                     /estimated_length=12
ORIGIN
        1 cnnnnnnnnn nntannnnag annnnctgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctaa gcttgggaga aggtccacca cggccgatat cacggccgtg
      121 gtggacggat ccaaatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
      181 ctctagagga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
      241 ttcctggaac aattgctttt acagatgcac atatcgaggt gaacatcacg tacgcggaat
      301 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
      361 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
      421 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
      481 acagtatgaa catttcgcag cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa
      541 ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg gattctaaaa
      601 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
      661 ttaatgaata cgattttgta ccagagtcct ttgatcgtga caaaacaatt gcactgataa
      721 tgaattcctc tggatctact gggttaccta agggtgtggc ccttccgcat agaactgcct
      781 gcgtcagnnn ctcgcatgcc agnnntccta tttttggcaa tcaaatcatt ccggnnactg
      841 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggnnatt
      901 tgatatgtgg nnnnnnngtn gncttaatgt atnnnnntga nnnnnnnnnn nnnnnannnt
      961 nnnntnagnn nnnnnnnnnn canannnnnt tgctagtncn aaccnnnnnn nca
//
