LOCUS       sequence-16698-e        1364 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3569
FEATURES             Location/Qualifiers
     source          1..1364
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             468..488
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(531..559)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgatcttact aactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca
       61 ctcacaacga tggatacgct aacaacttgg aaatgaaata agcttgcatg cctgcaggtc
      121 gactctagag gatccccaat tcttgagact tccactatct tctctggcac cctccactac
      181 gcgtaggcgt ctctcgcttc gtgtattccc gggaagccgg gttcccgtct ctcccgccgc
      241 tgccgctgcc gcacacagct ttacacctcg tagaatcccc aaagaggggc gtggcttgcg
      301 ggtgccaaca tcctcctgcc gaggaagaag caggcactca tcactcgcat catcaacctc
      361 gggattggcc aaaggaccca aaggtatgtt tcgaatgata ctaacataac atagaacatt
      421 ttcaggagga cccttggagg gtaccgagct cagaaaaaat gactgctcca aagaagaagc
      481 gtaaggtacc ggtagaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa
      541 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      601 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      661 ctgttccatg ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
      721 agccaacact tgtcactact ttcacttatg gtgttcaatg cttctcgaga tacccagatc
      781 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      841 ctatattttt caaagatgac gggaactaca agacacgtaa gtttaaacag ttcggtacta
      901 actaaccata catatttaaa ttttcaggtg ctgaagtcan gtttgaaggt gatacccttt
      961 gnttaataga atcgagttaa aaggtattga ttttaaaaaa anngaaacat tcttggacac
     1021 aantggaata cacttccact cccacaacgt atacntctgg canacaacca aaganggnat
     1081 caagntgtag ttaaactgat ttacnactac tancngatta atttccnant tcaattaacc
     1141 nccttganag gagngtccta cnacttntca caaattccnt gggaggncgt ntttncaaaa
     1201 acntacntcc caattgcntt taaancnaaa aaanaannng nctttgatnn aaccngggga
     1261 aacnggggga aataaaaaat tnaatcnngn gcgnnccctn cctttnggaa aaaggccntn
     1321 caaatacgtt caannaaatt ttcccccccc cccncncccc cccc
//
