LOCUS       sequence-166992-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses putidaredoxin reductase in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pPdR
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatgaacgca aacgacaacg
      121 tggtgattgt tggcactggt ctggctggcg ttgaagttgc gtttggtctg cgtgcaagcg
      181 gctgggaagg taacatccgc ctggtgggtg atgcgactgt tattccgcac cacctgccgc
      241 cgctgtctaa agcgtacctg gcgggcaagg cgactgcgga gtctctgtac ctgcgcactc
      301 cggatgctta cgcggctcag aacatccagc tgctgggcgg tacccaggtt accgcgatta
      361 accgtgaccg tcagcaggtg attctgtctg atggccgtgc actggattat gatcgtctgg
      421 tgctggcaac tggcggtcgt ccgcgtccgc tgccggtggc tagcggcgct gttggtaaag
      481 caaacaactt ccgttatctg cgtaccctgg aagacgcaga atgcatccgt cgccagctga
      541 ttgcggacaa ccgtctggtt gttatcggtg gcggctacat cggtctggag gttgctgcta
      601 ctgcgatcaa agcgaacatg cacgtgactc tgctggacac tgctgcgcgt gttctggaac
      661 gtgtgactgc tccgccggtg agcgcattct acgagcatct gcaccgcgaa gctggtgtgg
      721 acattcgtac tggtactcag gtttgcggct ttgaaatgtc taccgatcag cagaaagtta
      781 ctgcggttct gtgcgaagac ggcactcgtc tgccggctga tctggttatt gcgggtattg
      841 gcctgatccc gaactgcgaa ctggcatccg cagcgggcct gcaggttgac aacggcatcg
      901 tgatcaacga gcacatgcag actagcgatc cgctgattat ggcagtgggc gattgtgcgc
      961 gtttccactc tcagctgtat gaccgttggg ttcgcatcga aagcgtgccg aacgcgctgg
     1021 aacaggcgcg taaaatcg
//
