LOCUS       sequence-166994-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses putidaredoxin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pPdX
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             478..495
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(548..566)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      562..609
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      646..1026
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(733..752)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     943..964
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg tctaaagttg
      121 tttacgtttc tcacgacggt acccgtcgcg aactggacgt tgcggacggt gtttctctga
      181 tgcaggcggc agtttctaac ggcatctacg acatcgttgg tgactgcggt ggttctgcgt
      241 cttgcgcgac ctgccacgtg tacgtgaacg aagcgttcac cgacaaagtt ccggctgcta
      301 acgaacgtga aatcggtatg ctggaatctg ttaccgcgga actgaaaccg aactctcgtc
      361 tgtcttgcca gatcatcatg accccggagc tggacggtat cgttgttgac gttccggacc
      421 gtcagtggtg aggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac
      481 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      541 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      601 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
      661 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      721 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      781 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      841 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
      901 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
      961 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     1021 tttcggccta ttggttaaaa a
//
