LOCUS       sequence-166996-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses putidaredoxin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pPdX
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(426..443)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(453..470)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             489..511
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    526..550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(550..569)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(551..569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(653..672)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctc accactgacg gtccggaacg
      121 tcaacaacga taccgtccag ctccggggtc atgatgatct ggcaagacag acgagagttc
      181 ggtttcagtt ccgcggtaac agattccagc ataccgattt cacgttcgtt agcagccgga
      241 actttgtcgg tgaacgcttc gttcacgtac acgtggcagg tcgcgcaaga cgcagaacca
      301 ccgcagtcac caacgatgtc gtagatgccg ttagaaactg ccgcctgcat cagagaaaca
      361 ccgtccgcaa cgtccagttc gcgacgggta ccgtcgtgag aaacgtaaac aactttagac
      421 atatggctgc cgcgcggcac caggccgctg ctgtgatgat gatgatgatg gctgctgccc
      481 atggtatatc tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc
      541 tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
      601 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      661 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      721 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      781 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
      841 atgcaggagt cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac
      901 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg
//
