LOCUS       sequence-16700-e        1373 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3571
FEATURES             Location/Qualifiers
     source          1..1373
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             467..487
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(530..558)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
       61 tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgcatgc ctgcaggtcg
      121 actctagagg atccccaatt cttgagactt ccactatctt ctctggcacc ctccactacg
      181 cgtaggcgtc tctcgcttcg tgtattcccg ggaagccggg ttcccgtctc tcccgccgct
      241 gccgctgccg cacacagctt tacacctcgt agaatcccca aagaggggcg tggcttgcgg
      301 gtgccaacat cctcctgccg aggaagaagc aggcactcat cactcgcatc atcaacctcg
      361 ggattggcca aaggacccaa aggtatgttt cgaatgatac taacataaca tagaacattt
      421 tcaggaggac ccttggaggg taccgagctc agaaaaaatg actgctccaa agaagaagcg
      481 taaggtaccg gtagaaaaaa tgagtaaagg agaagaactt ttcactggag ttgtcccaat
      541 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      601 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      661 tgttccatgg gtaagtttaa acatatatat actaactaac cctgattatt taaattttca
      721 gccaacactt gtcactactt tcacttatgg tgttcaatgc ttctcgagat acccagatca
      781 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      841 tatatttttc aaagatgacg ggaactacaa gacacgtaag tttaaacagt tcggtactaa
      901 ctaaccatac atatttaaat tttcaggtgc tgaaagttca gtttggaagg ngataccctt
      961 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacnt tnctgggacn
     1021 caaattggaa taccaactat aactcccnca atgntnacct tgccaccaaa aaaccaaaga
     1081 atggnatcca agtngtaagt ttaacctgat ttnccnancn aacctantcg natttnaatt
     1141 tnnnaactnc aaattaaccc aactttgaan anggagggnt tcccnngccn ncctttncaa
     1201 aaaatctcca ttgggggagg ncctnttctt tnaaaaaaaa atnncggtnc cnaanttgcc
     1261 ntttaaaanc caanaaaaaa aaaccangnc ctttnnattn naacccnggg aatcccgggg
     1321 gggaaaatan aancctcnna agggnttann tncccaannn cccntttttt taa
//
