LOCUS       sequence-167000-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses P450cam (CYP101A1) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pP450cam
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..105
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc
       61 catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccatat gactaccgaa
      121 accatccagt ctaacgcgaa cctggctccg ctgccgccgc atgtgccgga acacctggtg
      181 ttcgacttcg atatgtacaa cccgtctaac ctgtctgcgg gcgttcagga agcgtgggcg
      241 gtgctgcagg aatccaacgt tccggatctg gtgtggaccc gctgcaacgg cggtcactgg
      301 atcgcgaccc gtggccagct gattcgtgaa gcatacgaag attaccgtca tttctcctct
      361 gaatgcccgt tcatcccgcg tgaggctggt gaggcgtacg atttcattcc gactagcatg
      421 gacccgccgg agcagcgcca gtttcgtgcg ctggcgaacc aggttgttgg tatgccggtt
      481 gtggataaac tggagaaccg tatccaggag ctggcgtgct ctctgattga gtctctgcgt
      541 ccgcagggtc agtgcaactt caccgaagac tacgcggaac cgtttccgat ccgtattttc
      601 atgctgctgg cgggtctgcc ggaggaagac attccgcacc tgaagtatct gaccgaccag
      661 atgactcgcc cggatggctc tatgaccttc gcggaggcga aagaagcgct gtacgactac
      721 ctgatcccga tcatcgaaca gcgccgtcag aagccgggta ccgacgcgat ctctatcgtt
      781 gcgaacggtc aggttaacgg tcgtccgatt acctccgatg aagcgaaacg tatgtgcggt
      841 ctgctgctgg tgggtggtct ggacactgtt gttaacttcc tgtctttctc tatgg
//
