LOCUS       sequence-167002-        1093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses P450cam (CYP101A1) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pP450cam
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctc aaaccgcttt agtggttgcc
      121 gggtcccaaa ccagcggcag agcctgcacg ccgctaacga taccagattt gtgctggatc
      181 tgcgcacccg gtgcaatgct gaaatccggg atacgagtca gccattcttt cagggtaaca
      241 atgatctcgc gacgcgccag gtgctggccc aggcacaggt gagagccgtg accgaaagta
      301 gtgtgagaaa ccttctggcg agaaaagtca acgtgcatcg gacacgcgtt ttcacgttcg
      361 tccaggccgc tcagcatctg cggcagcagg atctgatcac cttttttcag ctgcacaccg
      421 tggaactcgt agtcagaggt caggatgcga ccgtctgcaa ccagagagaa gcgacgcagc
      481 agttcttcgc acgcagccgg aatgcgctcc ggacgctcga tcagttcctg acgatgttcc
      541 gggcttttag ccagaaattc catagagaaa gacaggaagt taacaacagt gtccagacca
      601 cccaccagca gcagaccgca catacgtttc gcttcatcgg aggtaatcgg acgaccgtta
      661 acctgaccgt tcgcaacgat agagatcgcg tcggtacccg gcttctgacg gcgctgttcg
      721 atgatcggga tcaggtagtc gtacagcgct tctttcgcct ccgcgaaggt catagagcca
      781 tccgggcgag tcatctggtc ggtcagatac ttcaggtgcg gaatgtcttc ctccggcaga
      841 cccgccagca gcatgaaaat acggatcgga aacggttccg cgtagtcttc ggtgaagttg
      901 cactgaccct gcggacgcag agactcaatc agagagcacg ccagctcctg gatacggttc
      961 tccagtttat ccacaaccgg cataccaaca acctggttcg ccagcgcacg aaactggcgc
     1021 tgctccggcg ggtccatgct agtcggaatg aaatcgtacg cctcaccagc ctcacgcggg
     1081 atgaacgggc att
//
