LOCUS       sequence-167004-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and
            putidaredoxin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pR1G108CP20X
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg aacgcaaacg
      121 acaacgtggt gattgttggc actggtctgg ctggcgttga agttgcgttt ggtctgcgtg
      181 caagcggctg ggaaggtaac atccgcctgg tgggtgatgc gactgttatt ccgcaccacc
      241 tgccgccgct gtctaaagcg tacctggcgg gcaaggcgac tgcggagtct ctgtacctgc
      301 gcactccgga tgcttacgcg gctcagaaca tccagctgct gggcggtacc caggttaccg
      361 cgattaaccg tgaccgtcag caggtgattc tgtctgatgg ccgtgcactg gattatgatc
      421 gtctggtgct ggcaactggc ggtcgtccgc gtccgctgcc ggtggctagc ggcgctgttg
      481 gtaaagcaaa caacttccgt tatctgcgta ccctggaaga cgcagaatgc atccgtcgcc
      541 agctgattgc ggacaaccgt ctggttgtta tcggtggcgg ctacatcggt ctggaggttg
      601 ctgctactgc gatcaaagcg aacatgcacg tgactctgct ggacactgct gcgcgtgttc
      661 tggaacgtgt gactgctccg ccggtgagcg cattctacga gcatctgcac cgcgaagctg
      721 gtgtggacat tcgtactggt actcaggttt gcggctttga aatgtctacc gatcagcaga
      781 aagttactgc ggttctgtgc gaagacggca ctcgtctgcc ggctgatctg gttattgcgg
      841 gtattggcct gatcccgaac tgcgaactgg catccgcagc gggcctgcag gttgacaacg
      901 gcatcgtgat caacgagcac atgcagacta gcgatccgct gattatggca gtgggcgatt
      961 gtgcgcgttt ccactctcag ctgtatgacc gttgggttcg catcgaaagc gtgccgaacg
     1021 cgctggaaca ggcgcgtaaa atcgcagcaa tcctgtgcgg t
//
