LOCUS       sequence-167009-        6489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing STAG2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_STAG2_p.R541K
FEATURES             Location/Qualifiers
     source          1..6489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3889..3988)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4005..4024)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4006..4024)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4029..4045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4029..4045)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4476..5267
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5360..5948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5849..5868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6102..6119
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6278..6305)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6397..6483)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgatagca gctccagaaa taccaactga ttttaatcta ctacaggagt
      241 cagaaacaca tttttcttct gacacagatt ttgaagatat cgaaggaaaa aaccaaaagc
      301 aaggcaaagg caaaacttgt aaaaaaggca aaaagggccc agcagaaaag ggcaaaggtg
      361 gaaatggagg aggaaaacct ccttctggtc caaaccgaat gaatggtcat caccaacaga
      421 atggagtgga aaacatgatg ttgtttgaag ttgttaaaat gggcaagagt gctatgcagt
      481 cggtggtaga tgattggata gaatcataca agcatgaccg agatatagca cttcttgacc
      541 ttatcaactt ttttattcag tgttcaggct gtaaaggagt tgtcacagca gaaatgttta
      601 gacatatgca gaactctgag ataattcgaa aaatgactga agaattcgat gaggatagtg
      661 gagattatcc acttaccatg gctggtcctc agtggaagaa gttcaaatcc agtttttgtg
      721 aattcattgg cgtgttagta cggcaatgtc aatatagtat catatatgat gagtatatga
      781 tggatacagt catttcactt cttacaggat tgtctgactc acaagtcaga gcatttcgac
      841 atacaagcac cctggcagct atgaagttga tgacagcttt ggtgaatgtg gcactaaatc
      901 ttagcattaa tatggataat acacaaagac aatatgaagc agaacggaat aaaatgattg
      961 gaaaacgagc caatgagagg ctagaactcc tgctacaaaa gcggaaagag cttcaggaaa
     1021 atcaagatga aatagaaaat atgatgaatg caatatttaa aggagtgttt gtacatagat
     1081 accgtgatgc gatagctgaa attcgagcta tttgcattga agagattggc atttggatga
     1141 agatgtatag tgatgccttt cttaatgaca gttatttaaa atatgttggt tggactatgc
     1201 atgataagca aggtgaagta agactcaaat gtcttactgc tctacaaggg ctttattata
     1261 acaaagagct taattccaaa ctggaacttt ttaccagtcg gttcaaggat agaattgtgt
     1321 ctatgaccct tgacaaagaa tatgatgttg cagtacaagc aataaaatta ctcactcttg
     1381 ttttacagag tagtgaagaa gttctcactg cagaagattg tgaaaatgtc tatcatctgg
     1441 tttattcagc tcaccggcca gtagcagtag cagctggaga atttctctac aaaaagctct
     1501 tcagtcgtag agatccagag gaggatggaa tgatgaaaag aagaggaaga caaggtccaa
     1561 atgccaacct tgttaagaca ttggtttttt tctttctaga aagtgagtta catgagcatg
     1621 cagcatacct tgtggatagc atgtgggact gtgctactga gctgctgaaa gactgggaat
     1681 gtatgaatag cttgttactg gaagagccac ttagtggaga ggaagcacta acagataggc
     1741 aagagagtgc tctgattgaa ataatgcttt gtaccattag acaagcggct gaatgtcatc
     1801 ctcccgtggg aaaagggaca ggaaaaaggg tgcttacagc aaaggagaag aagacacagt
     1861 tggatgatag gacaaaaatc actgagcttt ttgccgtggc ccttcctcag ttattagcaa
     1921 aatactctgt agatgcagaa aaggtgacta acttgttgca gttgcctcag tactttgatt
     1981 tggaaatata taccactgga cgattagaaa agcatttgga tgccttattg cgacagatcc
     2041 ggaatattgt agagaagcac acagatacag atgttttgga agcatgttct aaaacttacc
     2101 atgcactctg taatgaagag ttcacaatct tcaacagagt agatatttca agaagtcaac
     2161 tgatagatga attggcagat aaatttaacc ggcttcttga agattttctg caagagggtg
     2221 aagaacctga tgaagatgat gcatatcagg tattgtcaac attgaagagg atcactgctt
     2281 ttcataatgc ccatgacctt tcaaagtggg atttatttgc ttgtaattac aaactcttga
     2341 aaactggaat cgaaaatgga gacatgcctg agcagattgt tattcacgca ctgcagtgta
     2401 ctcactatgt aatcctttgg caacttgcta agataactga aagcagctct acaaaggagg
     2461 acttgctgcg tttaaagaaa caaatgagag tattttgtca gatatgtcaa cattacctga
     2521 ccaacgtgaa tactactgtt aaggaacagg ccttcactat tctgtgtgat attttgatga
     2581 tcttcagcca tcagattatg tcaggagggc gtgacatgtt agagccatta gtgtataccc
     2641 ctgattcttc attgcagtct gagttgctaa gctttatttt ggatcatgtc ttcattgaac
     2701 aggatgatga taataatagt gcagatggtc agcaagagga tgaagccagt aaaattgaag
     2761 ctctgcacaa gagaagaaat ttacttgcag cattttgtaa gctaattgta tatactgtgg
     2821 tggagatgaa tacagctgca gatatcttca aacagtatat gaagtattat aatgactatg
     2881 gagatatcat caaagaaaca atgagtaaaa caaggcagat agacaaaatt cagtgtgcta
     2941 agacccttat tctcagtctg caacagcttt ttaatgaaat gatacaagaa aatggctata
     3001 attttgatag atcatcctct acatttagtg gcataaaaga acttgctcga cgttttgctt
     3061 taacttttgg acttgatcag ttgaaaacaa gagaagccat tgccatgcta cacaaagatg
     3121 gcatagaatt tgcttttaaa gagcctaatc cgcaagggga gagccatcca cctttaaatt
     3181 tggcatttct tgatattctg agtgaatttt cttctaaact acttcgacaa gacaaaagaa
     3241 cagtgtatgt ttacttggaa aagttcatga cctttcagat gtcactccga agagaggatg
     3301 tgtggcttcc actgatgtct taccgaaatt ctttgctagc tggtggtgat gatgacacca
     3361 tgtcagtcat tagtggaatc agcagccggg ggtcaacagt acggagtaaa aaatcaaaac
     3421 catctacagg aaaacggaaa gtggttgagg gcatgcagct ttcactcact gaagaaagta
     3481 gtagtagtga cagtatgtgg ttaagcagag aacaaacact gcacacccct gttatgatgc
     3541 agacaccaca actcacctcc actattatga gagagcccaa aagattacgg cctgaggata
     3601 gcttcatgag tgtttatcca atgcagactg aacatcatca aacacctctt gattataatc
     3661 ggcgtggcac aagcctaatg gaagatgatg aagagccaat tgtggaagat gttatgatgt
     3721 cctcagaagg gaggattgag gatcttaatg agggaatgga ttttgacacc atggatatag
     3781 atttgccacc atcaaagaac agacgagaga gaacagaact gaagcctgat ttctttgatc
     3841 cagcttcaat tatggatgaa tcagttcttg gagtgtcaat gttttgattg ccaactttct
     3901 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     3961 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     4021 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     4081 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     4141 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     4201 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     4261 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     4321 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     4381 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     4441 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     4501 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     4561 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     4621 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     4681 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     4741 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     4801 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     4861 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     4921 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     4981 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     5041 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     5101 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     5161 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     5221 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     5281 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     5341 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5401 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5461 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5521 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5581 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5641 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5701 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5761 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5821 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5881 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5941 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6001 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6061 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6121 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6181 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     6241 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     6301 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     6361 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     6421 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     6481 ttgatgcct
//
