LOCUS       sequence-167011-        4587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing WBSCR17 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_WBSCR17_p.D348N
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1987..2086)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2103..2122)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2104..2122)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2127..2143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2127..2143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2574..3365
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3458..4046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3947..3966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4200..4217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4376..4403)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4495..4581)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggcttcactg agaagagtca aagtgctgtt ggtgttgaac ttgatcgcgg
      241 tagccggctt cgtgctcttc ctggccaagt gccggcccat cgcggtgcgc agcggagacg
      301 ccttccacga gatccggccg cgcgccgagg tggccaacct cagcgcgcac agcgccagcc
      361 ccatccagga tgcggtcctg aagcgcctgt cgctgctgga ggacatcgtg taccggcagc
      421 tgaatggctt atccaaatcc cttgggctca ttgaaggtta tggtgggcgg ggtaaagggg
      481 gccttccggc tactctttcc ccggctgaag aagaaaaggc taagggaccc catgagaagt
      541 atggctacaa ttcatacctc agtgaaaaaa tttcactgga ccgttccatt ccggattatc
      601 gtcccaccaa gtgtaaggag ctcaagtact ccaaggacct gccccagata tccatcatat
      661 tcatcttcgt gaacgaggcc ctgtcggtga tcctgcggtc cgtgcacagt gccgtcaatc
      721 acacgcccac acacctgctg aaggaaatca ttctggtgga tgacaacagc gacgaagagg
      781 agctgaaggt ccccctagag gagtatgtcc acaaacgcta ccccgggctg gtgaaggtgg
      841 taagaaatca gaagagggaa ggcctgatcc gcgctcgcat tgagggctgg aaggtggcta
      901 ccgggcaggt cactggcttc tttgatgccc acgtggaatt caccgctggc tgggctgagc
      961 cggttctatc ccgcatccag gaaaaccgga agcgtgtgat cctcccctcc attgacaaca
     1021 tcaaacagga caactttgag gtgcagcggt acgagaactc ggcccacggg tacagctggg
     1081 agctgtggtg catgtacatc agccccccaa aagactggtg ggacgccgga gacccttctc
     1141 tccccatcag gaccccagcc atgataggct gctcgttcgt ggtcaacagg aagttcttcg
     1201 gtgaaattgg tcttctggat cctggcatga atgtatacgg aggagaaaat attgaactgg
     1261 gaatcaaggt atggctctgt gggggcagca tggaggtcct tccttgctca ctggtggccc
     1321 acattgagcg gaagaagaag ccatataata gcaacattgg cttctacacc aagaggaatg
     1381 ctcttcgcgt tgctgaggtc tggatggacg attacaagtc tcatgtgtac atagcgtgga
     1441 acctgccgct ggagaatccg ggaattgaca tcggtgatgt ctccgaaaga agagcattaa
     1501 ggaaaagttt aaagtgtaag aatttccagt ggtacctgga ccatgtttac ccagaaatga
     1561 gaagatacaa taataccgtt gcttacgggg agcttcgcaa caacaaggca aaagacgtct
     1621 gcttggacca ggggccgctg gagaaccaca cagcaatatt gtatccgtgc catggctggg
     1681 gaccacagct tgcccgctac accaaggaag gcttcctgca cttgggtgcc ctggggacca
     1741 ccacactcct ccccgacacc cgctgcctgg tggacaactc caagagtcgg ctgccccagc
     1801 tcctggactg cgacaaggtc aagagcagcc tgtacaagcg ctggaacttc atccagaatg
     1861 gagccatcat gaacaagggc acgggacgct gcctggaggt ggagaaccgg ggcctggctg
     1921 gcatcgacct catcctccgc agctgcacag gtcagaggtg gaccattaag aactccatca
     1981 agtgattgcc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     2041 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     2101 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     2161 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     2221 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     2281 cgtggaaacg gatggaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     2341 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     2401 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     2461 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2521 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     2581 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2641 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2701 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2761 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2821 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2881 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2941 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     3001 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     3061 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     3121 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3181 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3241 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3301 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     3361 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3421 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3481 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3541 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3601 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3661 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3721 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3781 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3841 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3901 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3961 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4021 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4081 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4141 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4201 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4261 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4321 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     4381 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4441 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4501 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4561 ctgagccttt cgttttattt gatgcct
//
