LOCUS       sequence-167016-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector containing a cassette for scarless insertion of sfGFP and 
            marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  TITLE     Kate O'Connor-Giles Lab Donor Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pScarlessHD-sfGFP-DsRed
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   43..77
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    352..473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(363..382)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     417..436
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(656..679)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aacctcctga accaccagaa cctcccgacc cgccggcctt aaccctagaa agatagtctg
       61 cgtaaaattg acgcatgcat tcttgaaata ttgctctctc tttctaaata gcgcgaatcc
      121 gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc
      181 aatgcggtaa gtgtcactga ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg
      241 attatctttt acgtgacttt taagatttaa ctcatacgat aattatattg ttatttcatg
      301 ttctacttac gtgataactt attatatata tattttcttg ttatagatat ctaagataca
      361 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      421 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
      481 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
      541 agtaaaacct ctacaaatgt ggtatggctg attatgatct agagtcgcgg ccgctacagg
      601 aacaggtggt ggcggccctc ggcgcgctcg tactgctcca cgatggtgta gtcctcgttg
      661 tgggaggtga tgtccagctt ggagtccacg tagtagtagc cgggcagctg cacgggcttc
      721 ttggccatgt agatggactt gaactccacc aggtagtggc cgccgtcctt cagcttcagg
      781 gccttgtgga tctcgccctt cagcacgccg tcgcgggggt acaggcgctc ggtggacgcc
      841 tcccagccca tagtcttctt ctgcattacg gg
//
