LOCUS       sequence-167017-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector containing a cassette for scarless insertion of sfGFP and 
            marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  TITLE     Kate O'Connor-Giles Lab Donor Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pScarlessHD-sfGFP-DsRed
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   543..605
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
       61 cgaactcgtg gccgttcacg gagccctcca tgcgcacctt gaagcgcatg aactccttga
      121 tgacgtcctc ggaggaggcc atggtggcga ccggcttcga gccgattgtt tagcttgttc
      181 agctgcgctt gtttatttgc ttagctttcg cttagcgacg tgttcacttt gcttgtttga
      241 attgaattgt cgctccgtag acgaagcgcc tctatttata ctccggcggt cgagggttcg
      301 aaatcgataa gcttggatcc taattgaatt agctctaatt gaattagtct ctaattgaat
      361 tagatcctaa aagttttgtt actttataga agaaattttg agtttttgtt tttttttaat
      421 aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata
      481 taataaaact taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa
      541 cacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc
      601 tagggttaat cttgtacagc tcatccatgc ccagggtgat gccggcggcg gtcacgaact
      661 ccagcagcac catgtgatcg cgcttctcgt tggggtcctt ggacagcacg ctctgggtgc
      721 tcaggtagtg gttatcgggc agcagcactg ggccgtcgcc gatgggggtg ttctgctggt
      781 agtgatcggc cagctgcacg gagccatcct ccacattgtg gcggatcttg aagttggcct
      841 tgatgccgtt c
//
