LOCUS       sequence-167018-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector containing a cassette for scarless insertion of 3xFLAG and 
            marked by the visible marker 3xP3-DsRed flanked by piggyBac 
            termini..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  TITLE     Kate O'Connor-Giles Lab Donor Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pScarlessHD-3xFLAG-DsRed
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..98
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     repeat_region   104..166
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      561..570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(697..715)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(747..767)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 ctggaggttc cggcggctca gggggtagta tggactacaa agaccatgac ggtgattata
       61 aagatcatga catcgattac aaggatgacg atgacaagat taaccctaga aagataatca
      121 tattgtgacg tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct
      181 gtatatcgag gtttatttat taatttgaat agatattaag ttttattata tttacactta
      241 catactaata ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa
      301 caaaaactca aaatttcttc tataaagtaa caaaactttt aggatctaat tcaattagag
      361 actaattcaa ttagagctaa ttcaattagg atccaagctt atcgatttcg aaccctcgac
      421 cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa
      481 agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa
      541 caatcggctc gaagccggtc gccaccatgg cctcctccga ggacgtcatc aaggagttca
      601 tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      661 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
      721 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt
      781 acgtgaagca ccccgccga
//
