LOCUS       sequence-167019-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector containing a cassette for scarless insertion of 3xFLAG and 
            marked by the visible marker 3xP3-DsRed flanked by piggyBac 
            termini..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     Kate O'Connor-Giles Lab Donor Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pScarlessHD-3xFLAG-DsRed
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   45..79
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    354..475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(365..384)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     419..438
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(658..681)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(856..875)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggaacctcct gaaccaccag aacctcccga cccgccggcc ttaaccctag aaagatagtc
       61 tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc tctttctaaa tagcgcgaat
      121 ccgtcgctgt gcatttagga catctcagtc gccgcttgga gctcccgtga ggcgtgcttg
      181 tcaatgcggt aagtgtcact gattttgaac tataacgacc gcgtgagtca aaatgacgca
      241 tgattatctt ttacgtgact tttaagattt aactcatacg ataattatat tgttatttca
      301 tgttctactt acgtgataac ttattatata tatattttct tgttatagat atctaagata
      361 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
      421 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
      481 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
      541 caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc ggccgctaca
      601 ggaacaggtg gtggcggccc tcggcgcgct cgtactgctc cacgatggtg tagtcctcgt
      661 tgtgggaggt gatgtccagc ttggagtcca cgtagtagta gccgggcagc tgcacgggct
      721 tcttggccat gtagatggac ttgaactcca ccaggtagtg gccgccgtcc ttcagcttca
      781 gggccttgtg gatctcgccc ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggacg
      841 cctcccagcc catagtcttc ttctgcatta cggggccgtc ggagggg
//
