LOCUS       sequence-167020-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector containing a cassette for scarless insertion of 2xHA and 
            marked by the visible marker 3xP3-DsRed flanked by piggyBac 
            termini..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  TITLE     Kate O'Connor-Giles Lab Donor Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pScarlessHD-2xHA-DsRed
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..59
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             63..89
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     repeat_region   95..157
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      552..561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(688..706)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(738..758)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 ctggaggttc cggcggctca gggggtagta tgtacccata cgatgttcca gattacgctg
       61 gatatccata tgatgttcca gattatgcta ttaaccctag aaagataatc atattgtgac
      121 gtacgttaaa gataatcatg cgtaaaattg acgcatgtgt tttatcggtc tgtatatcga
      181 ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt acatactaat
      241 aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc
      301 aaaatttctt ctataaagta acaaaacttt taggatctaa ttcaattaga gactaattca
      361 attagagcta attcaattag gatccaagct tatcgatttc gaaccctcga ccgccggagt
      421 ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac
      481 gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatcggct
      541 cgaagccggt cgccaccatg gcctcctccg aggacgtcat caaggagttc atgcgcttca
      601 aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      661 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
      721 ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc
      781 accccgccga catcc
//
