LOCUS       sequence-167021-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector containing a cassette for scarless insertion of 2xHA and 
            marked by the visible marker 3xP3-DsRed flanked by piggyBac 
            termini..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     Kate O'Connor-Giles Lab Donor Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pScarlessHD-2xHA-DsRed
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   39..73
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    348..469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(359..378)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     413..432
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(652..675)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(850..869)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcctgaacca ccagaacctc ccgacccgcc ggccttaacc ctagaaagat agtctgcgta
       61 aaattgacgc atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg
      121 ctgtgcattt aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg
      181 cggtaagtgt cactgatttt gaactataac gaccgcgtga gtcaaaatga cgcatgatta
      241 tcttttacgt gacttttaag atttaactca tacgataatt atattgttat ttcatgttct
      301 acttacgtga taacttatta tatatatatt ttcttgttat agatatctaa gatacattga
      361 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      421 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
      481 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
      541 aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc tacaggaaca
      601 ggtggtggcg gccctcggcg cgctcgtact gctccacgat ggtgtagtcc tcgttgtggg
      661 aggtgatgtc cagcttggag tccacgtagt agtagccggg cagctgcacg ggcttcttgg
      721 ccatgtagat ggacttgaac tccaccaggt agtggccgcc gtccttcagc ttcagggcct
      781 tgtggatctc gcccttcagc acgccgtcgc gggggtacag gcgctcggtg gacgcctccc
      841 agcccatagt cttcttctgc attacggggc cgtcggaggg gaagtt
//
