LOCUS       sequence-167022-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for generating dsDNA donors for HDR. Contains the visible 
            marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for 
            identifying backbone incorporation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  TITLE     Kate O'Connor-Giles Lab Donor Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pScarlessHD-DsRed-w+
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   544..606
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc
       61 tcgaactcgt ggccgttcac ggagccctcc atgcgcacct tgaagcgcat gaactccttg
      121 atgacgtcct cggaggaggc catggtggcg accggcttcg agccgattgt ttagcttgtt
      181 cagctgcgct tgtttatttg cttagctttc gcttagcgac gtgttcactt tgcttgtttg
      241 aattgaattg tcgctccgta gacgaagcgc ctctatttat actccggcgg tcgagggttc
      301 gaaatcgata agcttggatc ctaattgaat tagctctaat tgaattagtc tctaattgaa
      361 ttagatccta aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa
      421 taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat
      481 ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa
      541 acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt
      601 ctagggttaa gatcgcaggt gctgccacct gcgaattcgg ccccgaagac actataggtc
      661 tcctatacgt ctcttatacg acatcaccga tggggaaccc agacgctgag tacgtattct
      721 aaatgcataa taaatactga taacatctta tagtttgtat tatattttgt attatcgttg
      781 acatgtataa ttttgatatc aaaaactgat tttcccttta ttattttcga gatttatttt
      841 cttaattctc tttaacaaac tagaaatatt gtatatac
//
