LOCUS       sequence-167029-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for generating dsDNA donors for HDR. Contains the visible 
            marker 3xP3-DsRed and GMR-white for identifying backbone 
            incorporation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  TITLE     Kate O'Connor-Giles Lab Donor Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHD-DsRed-w+
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(380..413)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct
       61 cgatctcgaa ctcgtggccg ttcacggagc cctccatgcg caccttgaag cgcatgaact
      121 ccttgatgac gtcctcggag gaggccatgg tggcgaccgg cttcgagccg attgtttagc
      181 ttgttcagct gcgcttgttt atttgcttag ctttcgctta gcgacgtgtt cactttgctt
      241 gtttgaattg aattgtcgct ccgtagacga agcgcctcta tttatactcc ggcggtcgag
      301 ggttcgaaat cgataagctt ggatcctaat tgaattagct ctaattgaat tagtctctaa
      361 ttgaattaga tcccgtacga taacttcgta tagcatacat tatacgaagt tatgatcgca
      421 ggtgtgcata tgtccgcggc cgctagcatg caagaattcc acctgcttca gcgaagggcg
      481 acaccccata attagcccgg gcgaaaggcc cagtctttcg actgagcctt tcgttttatt
      541 tgatgcctgg cagttcccta ctctcgcatg gggagtcccc acactaccat cggcgctacg
      601 gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg
//
