LOCUS       sequence-167032-        5725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For construction of cDNA expression library..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Riesmeier JW, Willmitzer L, Frommer WB
  TITLE     Isolation and characterization of a sucrose carrier cDNA from 
            spinach by functional expression in yeast.
  JOURNAL   EMBO J. 1992 Dec;11(13):4705-13.
  PUBMED    1464305
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1992-12"; volume: "11(13)"; pages: "4705-13"
COMMENT     Sequence Label: p112A1NE
FEATURES             Location/Qualifiers
     source          1..5725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..407
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     terminator      594..781
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(649..673)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(793..810)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(793..809)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(802..824)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1246..2592
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(2704..3378)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(3379..3480)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(3500..3518)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3586..3690
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3691..3759,3760..4551)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3909..3928)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4722..5310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5211..5230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5464..5481
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5598..5619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5634..5639,5640..5657,5658..5664)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5672..5688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5677..5699
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5696..5712
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5696..5712
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agcttgcatg caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac
       61 catatccgca atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag
      301 acctgcaatt attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt
      361 tctttttctg cacaatattt caagctatac caagcataca atcaactcca agcttcagct
      421 gctgcagtct agagaattcc cgggcggccg cggatccagc tttggacttc ttcgccagag
      481 gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa
      541 agatggaaaa gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt
      601 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
      661 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
      721 aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg
      781 gcatgccaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      841 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      901 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
      961 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatcgga tcgtacttgt
     1021 tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac agatagtata
     1081 tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt atgtatttcg
     1141 gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc atccccggtt
     1201 cattttctgc gtttccatct tgcacttcaa tagcatatct ttgttaacga agcatctgtg
     1261 cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     1321 agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc
     1381 tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     1441 atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa
     1501 gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca
     1561 aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac
     1621 tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt
     1681 ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg
     1741 cattttttca agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac
     1801 tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt
     1861 ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt
     1921 cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga
     1981 taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg
     2041 ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg
     2101 tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt
     2161 tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata
     2221 ctttctagct agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag
     2281 cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat
     2341 atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc
     2401 ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg
     2461 tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg
     2521 ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt
     2581 tgatattgga tcgatccgat gataagctgt caaacatgag aattgatctt ttatgcttgc
     2641 ttttcaaaag gcttgcaggc aagtgcacaa acaatactta aataaatact actcagtaat
     2701 aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt
     2761 tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc
     2821 aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctctcgg ggctctcttg
     2881 ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc
     2941 tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt
     3001 gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc
     3061 ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat tgaccagagc
     3121 caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg gagtgcctga
     3181 actattttta tatgctttta caagacttga aattttcctt gcaataaccg ggtcaattgt
     3241 tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat ctagtgcaca
     3301 ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag aactacctgt
     3361 gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt
     3421 gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt cgaccgaatt
     3481 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3541 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3601 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3661 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3721 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3781 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3841 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3901 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3961 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4021 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4081 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4141 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4201 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4261 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4321 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4381 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4441 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4501 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4561 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4621 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4681 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4741 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4801 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4861 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4921 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4981 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5041 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5101 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5161 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5221 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5281 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5341 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5401 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5461 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     5521 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     5581 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     5641 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     5701 aacagctatg accatgatta cgcca
//
