LOCUS       sequence-167044-       11473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA845  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM845
FEATURES             Location/Qualifiers
     source          1..11473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        145..491
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     434..454
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    653..829
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1391..1409)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1392..1409)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2048..2067
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2170..2175,2176..2192,2193..2198))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2473..2853
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2859..3410
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3420..3672
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3758..3782
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4207..5001
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5088..5676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5830..5847
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5862..6002
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5928..5947)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6088..6110
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6346..6540
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6606..7679)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8108..8737)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10038..10062
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10250..10272
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10264..10281
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10265..10281
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10306..11013)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11192..11211
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11192..11210
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 catcgtggga aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat
       61 ctccactgac gtaagggatg acgcacaatc ccacccctac tccaagaatg tcaaagatac
      121 tgtctcagaa ggccaaaggg ctattgagac ttttcaacaa agggtaattt cgggaaacct
      181 cctcggattc cattgcccag ctatctgtca cctcatcgaa aggacagtag aaaaggaagg
      241 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atgcctctgc
      301 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      361 tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgaca taagggatga
      421 cgcacaaccc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      481 tggagaggac agcccaagct tcatcaattt ggtctaatca gagcgagttg ttgttgttat
      541 ggtctaattt aaatatggtc taaagaagaa gaatcatcaa ttggtctaat cagagccgga
      601 attcggtacg ctgaaatcac cagtctctct ctacaaatct atctctctct attttctcca
      661 taaataatgt gtgagtagtt tcccgataag ggaaattagg gttcttatag ggtttcgctc
      721 atgtgttgag catataagaa acccttagta tgtatttgta tctgtaaaat acttctatca
      781 ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatct cctaaagtcc
      841 ctatagatct ttgtcgtgaa tataaaccag acacgagacg actagacctg gagcccagac
      901 gccgttcgaa gctagaagta ccgcttaggc gggaggccgt tagggaaaag atgctaaggc
      961 agggttggtt acgttgactc ccccgtaggt ttggtttaaa tatgatgaag tggacggaag
     1021 gaaggaggaa gacaaggaag gataaggttg caggccctgt gcaaggtaag aagatggaaa
     1081 tttgatagag gtacgctact atacttatac tatacgctaa gggaatgatt gtatttatac
     1141 cctatacccc ctaataaccc cttatcaatt taagaaataa tccgcataag cccccgctta
     1201 aaaattggta tcagagccat gaataggtct atgaccaaaa ctcaagagga taaaacctca
     1261 ccaaaatacg aaagagtcct taactctaaa gataaaagat ctttcaagat caaaactagt
     1321 tccctcacac cggtgacggg gatcgcatgc gacctctaga ccatcacatt ggagcttcag
     1381 ctgctcgagt tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     1441 aattgtagcc gcgttcttac gtcgacgtac caattacgcc ccgccctgtc actcatcgca
     1501 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     1561 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1621 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1681 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     1741 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1801 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1861 acaagggtga acgctatccc atattaccag ctcaccgtct ttcattgcca tacggaactc
     1921 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1981 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     2041 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     2101 aacggtggta tatccagtga tttctttctc cattttagct tccttagctc ctgaaaatct
     2161 cgataactca ttaaagctta tcgatgataa gctgtcaaac atgagaattt agcccgccta
     2221 atgagcgggc tttttgttgt gatccgcgtt gctggcgttt ttccataggc tccgcccccc
     2281 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2341 aagataccag gcgtttcccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2401 cgcttaccgg atacctgtcc gcctttctta attaactctc tagactcacc taggatccat
     2461 gaattctgag atttttcaaa tcagtgcgca agacgtgacg taagtatccg agtcagtttt
     2521 tatttttcta ctaatttggt cgtttatttc ggcgtgtagg acatggcaac cgggcctgaa
     2581 tttcgcgggt attctgtttc tattccaact ttttcttgat ccgcagccat taacgacttt
     2641 tgaatagata cgctgacacg ccaagcctcg ctagtcaaaa gtgtaccaaa caacgcttta
     2701 cagcaagaac ggaatgcgcg tgacgctcgc ggtgacgcca tttcgccttt tcagaaatgg
     2761 ataaatagcc ttgcttccta ttatatcttc ccaaattacc aatacattac actagcatct
     2821 gaatttcata accaatctcg atacaccaaa tcgaatcgat gagcccagaa cgacgcccgg
     2881 ccgacatccg ccgtgccacc gaggcggaca tgccggcggt ctgcaccatc gtcaaccact
     2941 acatcgagac aagcacggtc aacttccgta ccgagccgca ggaaccgcag gagtggacgg
     3001 acgacctcgt ccgtctgcgg gagcgctatc cctggctcgt cgccgaggtg gacggcgagg
     3061 tcgccggcat cgcctacgcg ggcccctgga aggcacgcaa cgcctacgac tggacggccg
     3121 agtcgaccgt gtacgtctcc ccccgccacc agcggacggg actgggctcc acgctctaca
     3181 cccacctgct gaagtccctg gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc
     3241 tgcccaacga cccgagcgtg cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc
     3301 tgcgggcggc cggcttcaag cacgggaact ggcatgacgt gggtttctgg cagctggact
     3361 tcagcctgcc ggtaccgccc cgtccggtcc tgcccgtcac cgagatttga ctgcagaatc
     3421 ttggactccc atgttggcaa aggcaaccaa acaaacaatg aatgatccgc tcctgcatat
     3481 ggggcggttt gagtatttca actgccattt gggctgaatt gaagacatgc tcctgtcaga
     3541 aattccgtga tcttactcaa tattcagtaa tctcggccaa tatcctaaat gtgcgtggct
     3601 ttatctgtct ttgtattgtt tcatcaattc atgtaacgtt tgcttttctt atgaattttc
     3661 aaataaatta tcagatccga gctcgaatta attcggcgtt aattcagtac attaaaaacg
     3721 tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc
     3781 caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag
     3841 gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc
     3901 tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat
     3961 gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca
     4021 ccgcggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     4081 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt
     4141 cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa
     4201 taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc
     4261 tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat
     4321 gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa
     4381 cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac
     4441 tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc
     4501 tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc
     4561 atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc
     4621 cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac
     4681 tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa
     4741 aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg
     4801 aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg
     4861 tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc
     4921 gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt
     4981 ttactggatg aattgtttta gtacctagaa tgcatgacca aaatccctta acgtgagttt
     5041 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5101 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5161 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5221 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5281 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5341 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5401 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5461 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5521 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5581 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5641 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5701 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5761 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5821 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     5881 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     5941 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     6001 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     6061 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     6121 catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac
     6181 ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg
     6241 ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg
     6301 gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg
     6361 cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc
     6421 tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca
     6481 atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca
     6541 ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc
     6601 gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg
     6661 atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg
     6721 atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg
     6781 tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg
     6841 tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg
     6901 tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg
     6961 tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc
     7021 tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc
     7081 gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct
     7141 cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct
     7201 cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc
     7261 aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg
     7321 tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa
     7381 acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg
     7441 aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc
     7501 ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg
     7561 cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc
     7621 aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg
     7681 ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcaggca acccagccgc
     7741 ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg
     7801 gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt
     7861 catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc
     7921 ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag
     7981 ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg
     8041 cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct
     8101 cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag
     8161 cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc
     8221 tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg
     8281 caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta
     8341 gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc
     8401 atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg
     8461 acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga
     8521 tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat
     8581 cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa
     8641 catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg
     8701 acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt
     8761 atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac
     8821 acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc
     8881 cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg
     8941 cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat
     9001 gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca
     9061 ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc
     9121 cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag
     9181 cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc
     9241 tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct
     9301 cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac
     9361 tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc
     9421 cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc
     9481 agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca
     9541 gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc
     9601 ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag
     9661 tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc
     9721 atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg
     9781 acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc
     9841 gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag
     9901 cggaggggtt ggatcaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat
     9961 acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt attctaataa
    10021 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
    10081 tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagcattc gccattcagg
    10141 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    10201 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    10261 cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtcctgct gagcctcgac
    10321 atgttgtcgc aaaattcgcc ctggacccgc ccaacgattt gtcgtcactg tcaaggtttg
    10381 acctgcactt catttggggc ccacatacac caaaaaaatg ctgcataatt ctcggggcag
    10441 caagtcggtt acccggccgc cgtgctggac cgggttgaat ggtgcccgta actttcggta
    10501 gagcggacgg ccaatactca acttcaagga atctcaccca tgcgcgccgg cggggaaccg
    10561 gagttccctt cagtgagcgt tattagttcg ccgctcggtg tgtcgtagat actagcccct
    10621 ggggcacttt tgaaatttga ataagattta tgtaatcagt cttttaggtt tgaccggttc
    10681 tgccgctttt tttaaaattg gatttgtaat aataaaacgc aattgtttgt tattgtggcg
    10741 ctctatcata gatgtcgcta taaacctatt cagcacaata tattgttttc attttaatat
    10801 tgtacatata agtagtaggg tacaatcagt aaattgaacg gagaatatta ttcataaaaa
    10861 tacgatagta acgggtgata tattcattag aatgaaccga aaccggcggt aaggatctga
    10921 gctacacatg ctcaggtttt ttacaacgtg cacaacagaa ttgaaagcaa atatcatgcg
    10981 atcataggcg tctcgcatat ctcattaaag caggactcta gggactagtc ccgggtctta
    11041 attaagtttg caagcagcag attacgcgca gaaaaaaagg atctcaagga gatcctttgg
    11101 atcacaaaaa aaaagcccgc tcattaggcg ggttaaattc ccgggtactg cagccgattc
    11161 attaatgcag gttaacctgg cttatcgaaa ttaatacgac tcactatagg gagaccggca
    11221 gatctgatcc ctactccaaa aatgtcaaag atacagtctc agaagaccaa agggctattg
    11281 agacttttca acaaagggta atttcgggaa acctcctcgg attccattgc ccagctatct
    11341 gtcacttcat cgaaaggaca gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
    11401 ataaaggaaa ggctatcatt caagatgcct ctgccgacag tggtcccaaa gatggacccc
    11461 cacccacgag gag
//
