LOCUS       sequence-167046-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in
            E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pC3R112C/T180C
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg
       61 gccgcaagct tgtcgacgga gctcgaattc ggatctcaca ctttcggcgc cagcaggtac
      121 gtaactttgc caccgccttc catattgaaa aacagctgca gcggtttctg gttgccgaag
      181 ctgattttca cgtaatcact cagtttcgtc agagacagaa catcatccag gtattccgca
      241 ctgtaagaat ttttgctttc tttactaaat tccagatcct gcagaccgcc gcaatcttta
      301 gaaaattcca cttcaacttc gctttcgcct tctgctttga tcagaatgcg atcttcgtga
      361 ccttccacaa ccacattatc cgtcacggta gaaacttcgc tgatggcact tttaaaacca
      421 tcagagctga tcgtcgcact aatatcgaac tgcaggttga tttccggaat ttcctgttca
      481 gacacttcca gattgcaaac gttaaattca cgattggtgc tgccgataat gttgataatc
      541 acactatccg ggctttcact tgcgatttca atggcttctt tacgttttgc aactttcagg
      601 attttcatca ggtactgggt gttgaaaccg aatttaaatt catcgttcac atcgtattct
      661 ttgaacattt cgcgcggcag gttaacagag atcaggctaa tatgcgcacg atccagtgcc
      721 accagttcaa cgctatcctg tttgaatttc agcacggctt catcaaccag gcgggccagc
      781 gcctggataa tatctttcag cacacgaaca tcatcgtaaa ccactttcat attgatcagg
      841 cggatgttac gttcgaaaga tttcaggtag atcatggatc caccaaccgc tttagtggtt
      901 gccgggtccc aaaccagcgg cagagcctgc acgccgctaa cgataccaga tttgtgctgg
      961 atctgcgcac ccggtgcaat gctgaaatcc gggatacgag tcagccattc tttcagggta
     1021 acaatgatct c
//
